Description of Sp. Nov. and of Sp. Nov

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Nov 12

PMID 31708881

Citations 24

Authors

Cristina Merla

Carla Rodrigues

Virginie Passet

Marta Corbella

Harry A Thorpe

Teemu V S Kallonen

Zhiyong Zong

Piero Marone

Claudio Bandi

Davide Sassera

Jukka Corander

Edward J Feil

Sylvain Brisse

Affiliations

Soon will be listed here.

Abstract

causes opportunistic human infections and post-antibiotic haemorrhagic diarrhea. This species is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from, (Ko1), (Ko2), (Ko8), and (Ko6). The average nucleotide identity (ANI) of Ko3 and Ko4 were 90.7% with and 95.5% with , respectively. In addition, three strains of , a species so far described based on a single strain from a urinary tract infection patient in China, were isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry analysis allowed differentiating Ko3, Ko4, and Ko8 from the other species. Based on these results, we propose the names for the Ko3 phylogroup, with SPARK_775_C1 (CIP 111695 and DSM 109531) as type strain, and for Ko4, with SPARK_836_C1 (CIP 111696 and DSM 109530) as type strain. Strains of were isolated from human urine, cow feces, and farm surfaces, while strains of were found in fecal carriage from humans, cows, and turtles.

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