Strontium Enhances Proliferation and Osteogenic Behavior of Bone Marrow Stromal Cells of Mesenchymal and Ectomesenchymal Origins in Vitro

Overview

Journal Clin Exp Dent Res

Publisher Wiley

Specialty Dentistry

Date 2019 Nov 6

PMID 31687189

Citations 3

Authors

Carolina Bizelli-Silveira

Lisbeth Ann Abildtrup

Rubens Spin-Neto

Morten Foss

Kjeld Soballe

David Christian Evar Kraft

Affiliations

Soon will be listed here.

Abstract

Obejective: To investigate the effect of increasing Strontium (Sr) concentrations on the growth and osteogenic behavior of human bone marrow stromal cells (BMSCs) from mesenchymal (i.e., fibula) and ectomesenchymal (i.e., mandible) embryonic origins.

Materials And Methods: Fibula and mandible BMSCs were cultured in media without (Ctrl) or with Sr in four diverse concentrations: Sr1, 11.3 × 10 mg/L, human seric physiological level; Sr2, 13 mg/L, human seric level after strontium ranelate treatment; Sr3, 130 mg/L, and Sr4, 360 mg/L. Proliferation rate (1, 3, and 7 days), osteogenic behavior (alkaline phosphatase [ALP] activity, 7 and 14 days; expression of osteogenic genes (ALP, osteopontin, and osteocalcin at 7, 14, and 21 days), and formation of mineralized nodules (14 and 21 days) of the BMSCs were assessed. Data was compared group- and period-wise using analysis of variance tests.

Results: Fibula and mandible BMSCs cultured with Sr4 showed increased proliferation rate, and osteocalcin and osteopontin gene expression together with more evident formation of mineralized nodules, compared all other Sr concentrations. For both cell populations, Sr4 led to lower ALP activity, and ALP gene expression, compared with the other Sr concentrations.

Conclusion: BMSCs from mesenchymal (i.e., fibula) and ectomesenchymal (i.e., mandible) embryonic origins showed increased cellular proliferation and osteogenic behavior when cultured with Sr4, in vitro.

Citing Articles

Impact of Strontium, Magnesium, and Zinc Ions on the In Vitro Osteogenesis of Maxillary Sinus Membrane Stem Cells.

Zhang Z, Gong N, Wang Y, Xu L, Zhao S, Liu Y Biol Trace Elem Res. 2024; .

PMID: 39150638 DOI: 10.1007/s12011-024-04303-4.

The Role of Trace Elements and Minerals in Osteoporosis: A Review of Epidemiological and Laboratory Findings.

Skalny A, Aschner M, Silina E, Stupin V, Zaitsev O, Sotnikova T Biomolecules. 2023; 13(6).

PMID: 37371586 PMC: 10296274. DOI: 10.3390/biom13061006.

Strontium enhances proliferation and osteogenic behavior of bone marrow stromal cells of mesenchymal and ectomesenchymal origins in vitro.

Bizelli-Silveira C, Abildtrup L, Spin-Neto R, Foss M, Soballe K, Christian Evar Kraft D Clin Exp Dent Res. 2019; 5(5):541-550.

PMID: 31687189 PMC: 6820574. DOI: 10.1002/cre2.221.

References

Aimaiti A, Maimaitiyiming A, Boyong X, Aji K, Li C, Cui L . Low-dose strontium stimulates osteogenesis but high-dose doses cause apoptosis in human adipose-derived stem cells via regulation of the ERK1/2 signaling pathway. Stem Cell Res Ther. 2017; 8(1):282. PMC: 5735894. DOI: 10.1186/s13287-017-0726-8. View

Meunier P, Roux C, Seeman E, Ortolani S, Badurski J, Spector T . The effects of strontium ranelate on the risk of vertebral fracture in women with postmenopausal osteoporosis. N Engl J Med. 2004; 350(5):459-68. DOI: 10.1056/NEJMoa022436. View

Brennan T, Rybchyn M, Green W, Atwa S, Conigrave A, Mason R . Osteoblasts play key roles in the mechanisms of action of strontium ranelate. Br J Pharmacol. 2009; 157(7):1291-300. PMC: 2743848. DOI: 10.1111/j.1476-5381.2009.00305.x. View

Zhu L, Zaidi S, Peng Y, Zhou H, Moonga B, Blesius A . Induction of a program gene expression during osteoblast differentiation with strontium ranelate. Biochem Biophys Res Commun. 2007; 355(2):307-11. DOI: 10.1016/j.bbrc.2007.01.120. View

SHORR E, Carter A . The usefulness of strontium as an adjuvant to calcium in the remineralization of the skeleton in man. Bull Hosp Joint Dis. 1952; 13(1):59-66. View

Bizelli-Silveira C, Abildtrup L, Spin-Neto R, Foss M, Soballe K, Christian Evar Kraft D . Strontium enhances proliferation and osteogenic behavior of bone marrow stromal cells of mesenchymal and ectomesenchymal origins in vitro. Clin Exp Dent Res. 2019; 5(5):541-550. PMC: 6820574. DOI: 10.1002/cre2.221. View

Bonnelye E, Chabadel A, Saltel F, Jurdic P . Dual effect of strontium ranelate: stimulation of osteoblast differentiation and inhibition of osteoclast formation and resorption in vitro. Bone. 2007; 42(1):129-38. DOI: 10.1016/j.bone.2007.08.043. View

Guise T, Mundy G . Cancer and bone. Endocr Rev. 1998; 19(1):18-54. DOI: 10.1210/edrv.19.1.0323. View

Beertsen W, van den Bos T . Alkaline phosphatase induces the mineralization of sheets of collagen implanted subcutaneously in the rat. J Clin Invest. 1992; 89(6):1974-80. PMC: 295899. DOI: 10.1172/JCI115805. View

10.

Luu Y, Capilla E, Rosen C, Gilsanz V, Pessin J, Judex S . Mechanical stimulation of mesenchymal stem cell proliferation and differentiation promotes osteogenesis while preventing dietary-induced obesity. J Bone Miner Res. 2008; 24(1):50-61. PMC: 2689082. DOI: 10.1359/jbmr.080817. View

11.

Gronthos S, Akintoye S, Wang C, Shi S . Bone marrow stromal stem cells for tissue engineering. Periodontol 2000. 2006; 41:188-95. DOI: 10.1111/j.1600-0757.2006.00154.x. View

12.

Simonds W, Agarwal S, Yang B, Skarulis M, Hendy G, Marx S . Familial isolated hyperparathyroidism: clinical and genetic characteristics of 36 kindreds. Medicine (Baltimore). 2002; 81(1):1-26. DOI: 10.1097/00005792-200201000-00001. View

13.

Bizelli-Silveira C, Pullisaar H, Abildtrup L, Andersen O, Spin-Neto R, Foss M . Strontium enhances proliferation and osteogenic behavior of periodontal ligament cells in vitro. J Periodontal Res. 2018; 53(6):1020-1028. DOI: 10.1111/jre.12601. View

14.

FRIEDENSTEIN A, Latzinik N, Grosheva A, Gorskaya U . Marrow microenvironment transfer by heterotopic transplantation of freshly isolated and cultured cells in porous sponges. Exp Hematol. 1982; 10(2):217-27. View

15.

Canalis E, Hott M, Deloffre P, Tsouderos Y, Marie P . The divalent strontium salt S12911 enhances bone cell replication and bone formation in vitro. Bone. 1996; 18(6):517-23. DOI: 10.1016/8756-3282(96)00080-4. View

16.

Choudhary S, Halbout P, Alander C, Raisz L, Pilbeam C . Strontium ranelate promotes osteoblastic differentiation and mineralization of murine bone marrow stromal cells: involvement of prostaglandins. J Bone Miner Res. 2007; 22(7):1002-10. DOI: 10.1359/jbmr.070321. View

17.

Aghaloo T, Chaichanasakul T, Bezouglaia O, Kang B, Franco R, Dry S . Osteogenic potential of mandibular vs. long-bone marrow stromal cells. J Dent Res. 2010; 89(11):1293-8. PMC: 3113466. DOI: 10.1177/0022034510378427. View

18.

Krebsbach P, Kuznetsov S, Bianco P, Robey P . Bone marrow stromal cells: characterization and clinical application. Crit Rev Oral Biol Med. 2000; 10(2):165-81. DOI: 10.1177/10454411990100020401. View

19.

Park J, Kim Y, Jang J, Song H . Positive modulation of osteogenesis- and osteoclastogenesis-related gene expression with strontium-containing microstructured Ti implants in rabbit cancellous bone. J Biomed Mater Res A. 2012; 101(1):298-306. DOI: 10.1002/jbm.a.34433. View

20.

Bianco P, Riminucci M, Gronthos S, Robey P . Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells. 2001; 19(3):180-92. DOI: 10.1634/stemcells.19-3-180. View