Reduction-Triggered Paclitaxel Release Nano-Hybrid System Based on Core-Crosslinked Polymer Dots with a PH-Responsive Shell-Cleavable Colorimetric Biosensor

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Oct 31

PMID 31661903

Citations 7

Authors

Seul Gi Kim

Benny Ryplida

Pham Thi My Phuong

Hyun Jeong Won

Gibaek Lee

Suk Ho Bhang

Sung Young Park

Affiliations

Soon will be listed here.

Abstract

Herein, we describe the fabrication and characterization of carbonized disulfide core-crosslinked polymer dots with pH-cleavable colorimetric nanosensors, based on diol dye-conjugated fluorescent polymer dots (L-PD), for reduction-triggered paclitaxel (PTX) release during fluorescence imaging-guided chemotherapy of tumors. L-PD were loaded with PTX (PTX loaded L-PD), via π-π stackings or hydrophobic interactions, for selective theragnosis by enhanced release of PTX after the cleavage of disulfide bonds by high concentration of glutathione (GSH) in a tumor. The nano-hybrid system showed fluorescence quenching behavior with less than 2% of PTX released under physiological conditions. However, in a tumor microenvironment, the fluorescence recovered at an acidic-pH, and PTX (approximately 100% of the drug release) was released efficiently out of the matrix by reduction caused by the GSH level in the tumor cells, which improved the effectiveness of the cancer treatment. Therefore, the colorimetric nanosensor showed promising potential in distinguishing between normal and cancerous tissues depending on the surrounding pH and GSH concentrations so that PTX can be selectively delivered into cancer cells for improved cancer diagnosis and chemotherapy.

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References

Senapati S, Mahanta A, Kumar S, Maiti P . Controlled drug delivery vehicles for cancer treatment and their performance. Signal Transduct Target Ther. 2018; 3:7. PMC: 5854578. DOI: 10.1038/s41392-017-0004-3. View

Li S, Hu K, Cao W, Sun Y, Sheng W, Li F . pH-responsive biocompatible fluorescent polymer nanoparticles based on phenylboronic acid for intracellular imaging and drug delivery. Nanoscale. 2014; 6(22):13701-9. DOI: 10.1039/c4nr04054f. View

Li Y, Xiao W, Xiao K, Berti L, Luo J, Tseng H . Well-defined, reversible boronate crosslinked nanocarriers for targeted drug delivery in response to acidic pH values and cis-diols. Angew Chem Int Ed Engl. 2012; 51(12):2864-9. PMC: 3545653. DOI: 10.1002/anie.201107144. View

Basiruddin S, Swain S . Phenylboronic acid functionalized reduced graphene oxide based fluorescence nano sensor for glucose sensing. Mater Sci Eng C Mater Biol Appl. 2015; 58:103-9. DOI: 10.1016/j.msec.2015.07.068. View

Lee M, Yang Z, Lim C, Lee Y, Dongbang S, Kang C . Disulfide-cleavage-triggered chemosensors and their biological applications. Chem Rev. 2013; 113(7):5071-109. DOI: 10.1021/cr300358b. View

Wang X, Xia N, Liu L . Boronic Acid-based approach for separation and immobilization of glycoproteins and its application in sensing. Int J Mol Sci. 2013; 14(10):20890-912. PMC: 3821649. DOI: 10.3390/ijms141020890. View

Wang Z, Deng X, Ding J, Zhou W, Zheng X, Tang G . Mechanisms of drug release in pH-sensitive micelles for tumour targeted drug delivery system: A review. Int J Pharm. 2017; 535(1-2):253-260. DOI: 10.1016/j.ijpharm.2017.11.003. View

Shi Y, Lammers T, Storm G, Hennink W . Physico-Chemical Strategies to Enhance Stability and Drug Retention of Polymeric Micelles for Tumor-Targeted Drug Delivery. Macromol Biosci. 2016; 17(1). PMC: 5410994. DOI: 10.1002/mabi.201600160. View

Parveen S, Misra R, Sahoo S . Nanoparticles: a boon to drug delivery, therapeutics, diagnostics and imaging. Nanomedicine. 2011; 8(2):147-66. DOI: 10.1016/j.nano.2011.05.016. View

10.

Liu D, Yang F, Xiong F, Gu N . The Smart Drug Delivery System and Its Clinical Potential. Theranostics. 2016; 6(9):1306-23. PMC: 4924501. DOI: 10.7150/thno.14858. View

11.

Zhao L, Zhao L, Miao Y, Liu C, Zhang C . Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity. J Anal Methods Chem. 2016; 2016:4306838. PMC: 5059578. DOI: 10.1155/2016/4306838. View

12.

Choi C, Mazrad Z, Lee G, In I, Lee K, Park S . Boronate-based fluorescent carbon dot for rapid and selectively bacterial sensing by luminescence off/on system. J Pharm Biomed Anal. 2018; 159:1-10. DOI: 10.1016/j.jpba.2018.06.043. View

13.

Lv H, Zhen C, Liu J, Yang P, Hu L, Shang P . Unraveling the Potential Role of Glutathione in Multiple Forms of Cell Death in Cancer Therapy. Oxid Med Cell Longev. 2019; 2019:3150145. PMC: 6590529. DOI: 10.1155/2019/3150145. View

14.

Su Z, Xu Y, Wang Y, Shi W, Han S, Shuai X . A pH and reduction dual-sensitive polymeric nanomicelle for tumor microenvironment triggered cellular uptake and controlled intracellular drug release. Biomater Sci. 2019; 7(9):3821-3831. DOI: 10.1039/c9bm00825j. View

15.

Estrella V, Chen T, Lloyd M, Wojtkowiak J, Cornnell H, Ibrahim-Hashim A . Acidity generated by the tumor microenvironment drives local invasion. Cancer Res. 2013; 73(5):1524-35. PMC: 3594450. DOI: 10.1158/0008-5472.CAN-12-2796. View

16.

Guo X, Cheng Y, Zhao X, Luo Y, Chen J, Yuan W . Advances in redox-responsive drug delivery systems of tumor microenvironment. J Nanobiotechnology. 2018; 16(1):74. PMC: 6151045. DOI: 10.1186/s12951-018-0398-2. View

17.

Wang S, Wang H, Liu Z, Wang L, Wang X, Su L . Smart pH- and reduction-dual-responsive folate-PEG-coated polymeric lipid vesicles for tumor-triggered targeted drug delivery. Nanoscale. 2014; 6(13):7635-42. DOI: 10.1039/c4nr00843j. View

18.

Chen H, Liu C, Xia Y . One-step synthesis of boronic acid functionalized gold nanoclusters for photoluminescence sensing of dopamine. Methods Appl Fluoresc. 2017; 5(1):014006. DOI: 10.1088/2050-6120/aa5e2d. View

19.

Cui P, Zhuang W, Hu X, Xing L, Yu R, Qiao J . A new strategy for hydrophobic drug delivery using a hydrophilic polymer equipped with stacking units. Chem Commun (Camb). 2018; 54(59):8218-8221. DOI: 10.1039/c8cc04363a. View

20.

Yu B, Meng Q, Hu H, Xu T, Shen Y, Cong H . Construction of Dimeric Drug-Loaded Polymeric Micelles with High Loading Efficiency for Cancer Therapy. Int J Mol Sci. 2019; 20(8). PMC: 6515377. DOI: 10.3390/ijms20081961. View