Principles and Applications of Optogenetics in Developmental Biology

Overview

Journal Development

Specialty Biology

Date 2019 Oct 24

PMID 31641044

Citations 48

Authors

Daniel Krueger

Emiliano Izquierdo

Ranjith Viswanathan

Jonas Hartmann

Cristina Pallares Cartes

Stefano De Renzis

Affiliations

Soon will be listed here.

Abstract

The development of multicellular organisms is controlled by highly dynamic molecular and cellular processes organized in spatially restricted patterns. Recent advances in optogenetics are allowing protein function to be controlled with the precision of a pulse of laser light , providing a powerful new tool to perturb developmental processes at a wide range of spatiotemporal scales. In this Primer, we describe the most commonly used optogenetic tools, their application in developmental biology and in the nascent field of synthetic morphogenesis.

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References

Viswanathan R, Necakov A, Trylinski M, Harish R, Krueger D, Esposito E . Optogenetic inhibition of Delta reveals digital Notch signalling output during tissue differentiation. EMBO Rep. 2019; 20(12):e47999. PMC: 6893285. DOI: 10.15252/embr.201947999. View

Wu Y, Frey D, Lungu O, Jaehrig A, Schlichting I, Kuhlman B . A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009; 461(7260):104-8. PMC: 2766670. DOI: 10.1038/nature08241. View

Crick F . The impact of molecular biology on neuroscience. Philos Trans R Soc Lond B Biol Sci. 2000; 354(1392):2021-5. PMC: 1692710. DOI: 10.1098/rstb.1999.0541. View

Guglielmi G, Barry J, Huber W, De Renzis S . An Optogenetic Method to Modulate Cell Contractility during Tissue Morphogenesis. Dev Cell. 2016; 35(5):646-660. PMC: 4683098. DOI: 10.1016/j.devcel.2015.10.020. View

Levskaya A, Weiner O, Lim W, Voigt C . Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009; 461(7266):997-1001. PMC: 2989900. DOI: 10.1038/nature08446. View

Motta-Mena L, Reade A, Mallory M, Glantz S, Weiner O, Lynch K . An optogenetic gene expression system with rapid activation and deactivation kinetics. Nat Chem Biol. 2014; 10(3):196-202. PMC: 3944926. DOI: 10.1038/nchembio.1430. View

Lee S, Park H, Kyung T, Kim N, Kim S, Kim J . Reversible protein inactivation by optogenetic trapping in cells. Nat Methods. 2014; 11(6):633-6. DOI: 10.1038/nmeth.2940. View

Banghart M, Borges K, Isacoff E, Trauner D, Kramer R . Light-activated ion channels for remote control of neuronal firing. Nat Neurosci. 2004; 7(12):1381-6. PMC: 1447674. DOI: 10.1038/nn1356. View

Rullan M, Benzinger D, Schmidt G, Milias-Argeitis A, Khammash M . An Optogenetic Platform for Real-Time, Single-Cell Interrogation of Stochastic Transcriptional Regulation. Mol Cell. 2018; 70(4):745-756.e6. PMC: 5971206. DOI: 10.1016/j.molcel.2018.04.012. View

10.

De Calisto J, Araya C, Marchant L, Riaz C, Mayor R . Essential role of non-canonical Wnt signalling in neural crest migration. Development. 2005; 132(11):2587-97. DOI: 10.1242/dev.01857. View

11.

Glantz S, Carpenter E, Melkonian M, Gardner K, Boyden E, Wong G . Functional and topological diversity of LOV domain photoreceptors. Proc Natl Acad Sci U S A. 2016; 113(11):E1442-51. PMC: 4801262. DOI: 10.1073/pnas.1509428113. View

12.

Wang X, He L, Wu Y, Hahn K, Montell D . Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nat Cell Biol. 2010; 12(6):591-7. PMC: 2929827. DOI: 10.1038/ncb2061. View

13.

Yizhar O, Fenno L, Davidson T, Mogri M, Deisseroth K . Optogenetics in neural systems. Neuron. 2011; 71(1):9-34. DOI: 10.1016/j.neuron.2011.06.004. View

14.

Takahashi F, Yamagata D, Ishikawa M, Fukamatsu Y, Ogura Y, Kasahara M . AUREOCHROME, a photoreceptor required for photomorphogenesis in stramenopiles. Proc Natl Acad Sci U S A. 2007; 104(49):19625-30. PMC: 2148339. DOI: 10.1073/pnas.0707692104. View

15.

Wolpert L . Positional information and the spatial pattern of cellular differentiation. J Theor Biol. 1969; 25(1):1-47. DOI: 10.1016/s0022-5193(69)80016-0. View

16.

Zayner J, Antoniou C, Sosnick T . The amino-terminal helix modulates light-activated conformational changes in AsLOV2. J Mol Biol. 2012; 419(1-2):61-74. PMC: 3338903. DOI: 10.1016/j.jmb.2012.02.037. View

17.

Jung H, Kim S, Kim M, Hong J, Yu D, Kim J . Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions. Nat Commun. 2019; 10(1):314. PMC: 6338782. DOI: 10.1038/s41467-018-08282-8. View

18.

Krueger D, Quinkler T, Mortensen S, Sachse C, De Renzis S . Cross-linker-mediated regulation of actin network organization controls tissue morphogenesis. J Cell Biol. 2019; 218(8):2743-2761. PMC: 6683744. DOI: 10.1083/jcb.201811127. View

19.

Meshik X, ONeill P, Gautam N . Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol. 2019; 8(3):498-510. PMC: 7081452. DOI: 10.1021/acssynbio.8b00356. View

20.

Sako K, Pradhan S, Barone V, Ingles-Prieto A, Muller P, Ruprecht V . Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation. Cell Rep. 2016; 16(3):866-77. DOI: 10.1016/j.celrep.2016.06.036. View