LncRNA PCAT6 Increased Cholangiocarcinoma Cell Proliferation and Invasion Via Modulating MiR-330-5p

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2019 Oct 22

PMID 31632586

Citations 16

Authors

Yongjie Xin

Xu He

Wei Zhao

Meixiao Zhan

Yong Li

Jing Xiao

Ke He

Ligong Lu

Affiliations

Soon will be listed here.

Abstract

Long non-coding RNAs (lncRNAs) act important roles in several tumors including cholangiocarcinoma. However, the expression pattern and function of PCAT6 in intrahepatic cholangiocarcinoma remains unknown. In our research, we showed that the PCAT6 expression level was upregulated in intrahepatic cholangiocarcinoma cell lines. The expression of PCAT6 in intrahepatic cholangiocarcinoma tissues than that in noncancerous samples and the higer expression of PCAT6 was associated with advanced stage. Ectopic expression of PCAT6 induced cell proliferation and invasion in cholangiocarcinoma cell. Moreover, we demonstrated that PCAT6 interacts with miR-330-5p by directly targeting and PCAT6 overexpression inhibited the expression of miR-330-5p in the ICC-9810 cell. We also showed that the expression level of miR-330-5p in intrahepatic cholangiocarcinoma samples was downregulated compared to noncancerous tissues. Interesting, we proved that the miR-330-5p expression was negative correlated with PCAT6 expression in intrahepatic cholangiocarcinoma. Ectopic expression of miR-330-5p suppressed cell proliferation and invasion. Finally, we showed that PCAT6 induced cell proliferation and invasion by decreasing miR-330-5p in cholangiocarcinoma cell. Taken together, these data suggested that lncRNA PCAT6 was an oncogenic player in the development of intrahepatic cholangiocarcinoma.

Citing Articles

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References

Trehoux S, Lahdaoui F, Delpu Y, Renaud F, Leteurtre E, Torrisani J . Micro-RNAs miR-29a and miR-330-5p function as tumor suppressors by targeting the MUC1 mucin in pancreatic cancer cells. Biochim Biophys Acta. 2015; 1853(10 Pt A):2392-403. DOI: 10.1016/j.bbamcr.2015.05.033. View

Huang W, Su G, Huang X, Zou A, Wu J, Yang Y . Long noncoding RNA PCAT6 inhibits colon cancer cell apoptosis by regulating anti-apoptotic protein ARC expression via EZH2. Cell Cycle. 2018; 18(1):69-83. PMC: 6343696. DOI: 10.1080/15384101.2018.1558872. View

Li Q, Shen W, Li X, Zhang L, Jin X . The lncRNA n340790 accelerates carcinogenesis of thyroid cancer by regulating miR-1254. Am J Transl Res. 2017; 9(5):2181-2194. PMC: 5446502. View

Wang S, Yin J, Li T, Yuan L, Wang D, He J . Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma. Oncol Rep. 2014; 33(2):819-25. DOI: 10.3892/or.2014.3641. View

Kunkeaw N, Jeon S, Lee K, Johnson B, Tanasanvimon S, Javle M . Cell death/proliferation roles for nc886, a non-coding RNA, in the protein kinase R pathway in cholangiocarcinoma. Oncogene. 2012; 32(32):3722-31. PMC: 3869796. DOI: 10.1038/onc.2012.382. View

Li J, Tian F, Li D, Chen J, Jiang P, Zheng S . MiR-605 represses PSMD10/Gankyrin and inhibits intrahepatic cholangiocarcinoma cell progression. FEBS Lett. 2014; 588(18):3491-500. DOI: 10.1016/j.febslet.2014.08.008. View

Du Z, Fei T, Verhaak R, Su Z, Zhang Y, Brown M . Integrative genomic analyses reveal clinically relevant long noncoding RNAs in human cancer. Nat Struct Mol Biol. 2013; 20(7):908-13. PMC: 3702647. DOI: 10.1038/nsmb.2591. View

Li B, Han Q, Zhu Y, Yu Y, Wang J, Jiang X . Down-regulation of miR-214 contributes to intrahepatic cholangiocarcinoma metastasis by targeting Twist. FEBS J. 2012; 279(13):2393-8. DOI: 10.1111/j.1742-4658.2012.08618.x. View

Zhang Q, Jeang K . Long non-coding RNAs (lncRNAs) and viral infections. Biomed Pharmacother. 2013; 3(1):34-42. PMC: 3641704. DOI: 10.1016/j.biomed.2013.01.001. View

10.

Huang J, Ma L, Song W, Lu B, Huang Y, Dong H . LncRNA-MALAT1 Promotes Angiogenesis of Thyroid Cancer by Modulating Tumor-Associated Macrophage FGF2 Protein Secretion. J Cell Biochem. 2017; 118(12):4821-4830. DOI: 10.1002/jcb.26153. View

11.

Yamanaka S, Campbell N, An F, Kuo S, Potter J, Mezey E . Coordinated effects of microRNA-494 induce G₂/M arrest in human cholangiocarcinoma. Cell Cycle. 2012; 11(14):2729-38. PMC: 3409013. DOI: 10.4161/cc.21105. View

12.

Meng F, Henson R, Lang M, Wehbe H, Maheshwari S, Mendell J . Involvement of human micro-RNA in growth and response to chemotherapy in human cholangiocarcinoma cell lines. Gastroenterology. 2006; 130(7):2113-29. DOI: 10.1053/j.gastro.2006.02.057. View

13.

Wan L, Zhang L, Fan K, Cheng Z, Sun Q, Wang J . Knockdown of Long Noncoding RNA PCAT6 Inhibits Proliferation and Invasion in Lung Cancer Cells. Oncol Res. 2016; 24(3):161-70. PMC: 7838661. DOI: 10.3727/096504016X14618564639178. View

14.

Haga H, Yan I, Takahashi K, Wood J, Patel T . Emerging insights into the role of microRNAs in the pathogenesis of cholangiocarcinoma. Gene Expr. 2014; 16(2):93-9. PMC: 4166576. DOI: 10.3727/105221614X13919976902174. View

15.

Wang X, Lu X, Geng Z, Yang G, Shi Y . LncRNA PTCSC3/miR-574-5p Governs Cell Proliferation and Migration of Papillary Thyroid Carcinoma via Wnt/β-Catenin Signaling. J Cell Biochem. 2017; 118(12):4745-4752. DOI: 10.1002/jcb.26142. View

16.

Chusorn P, Namwat N, Loilome W, Techasen A, Pairojkul C, Khuntikeo N . Overexpression of microRNA-21 regulating PDCD4 during tumorigenesis of liver fluke-associated cholangiocarcinoma contributes to tumor growth and metastasis. Tumour Biol. 2013; 34(3):1579-88. DOI: 10.1007/s13277-013-0688-0. View

17.

Su B, Zhou S, Gan C, Zhang X . MiR-330-5p regulates tyrosinase and PDIA3 expression and suppresses cell proliferation and invasion in cutaneous malignant melanoma. J Surg Res. 2016; 203(2):434-40. DOI: 10.1016/j.jss.2016.03.021. View

18.

Liu J, Song Z, Feng C, Lu Y, Zhou Y, Lin Y . The long non-coding RNA SUMO1P3 facilitates breast cancer progression by negatively regulating miR-320a. Am J Transl Res. 2018; 9(12):5594-5602. PMC: 5752909. View

19.

Ma X, Qi S, Duan Z, Liao H, Yang B, Wang W . Long non-coding RNA LOC554202 modulates chordoma cell proliferation and invasion by recruiting EZH2 and regulating miR-31 expression. Cell Prolif. 2017; 50(6). PMC: 6529120. DOI: 10.1111/cpr.12388. View

20.

Iwaki J, Kikuchi K, Mizuguchi Y, Kawahigashi Y, Yoshida H, Uchida E . MiR-376c down-regulation accelerates EGF-dependent migration by targeting GRB2 in the HuCCT1 human intrahepatic cholangiocarcinoma cell line. PLoS One. 2013; 8(7):e69496. PMC: 3724868. DOI: 10.1371/journal.pone.0069496. View