Isolation of Phytochemicals from L. Bark and Their In Vitro Antioxidant and Cytotoxic Potential
Overview
Authors
Affiliations
Plants have been the basis of traditional medicine since the dawn of civilizations. Different plant parts possess various phytochemicals, playing important roles in preventing and curing diseases. Scientists, through extensive experimental studies, are playing an important part in establishing the use of phytochemicals in medicine. However, there are still a large number of medicinal plants which need to be studied for their phytochemical profile. In this study, the objective was to isolate phytochemicals from bark of L. and to study them for their antioxidant and cytotoxic activities. The bark was extracted with methanol, followed by column chromatography and thus isolating kaempferol, stigmasterol, protocatechuic acid-methyl ester (PCA-ME) and protocatechuic acid (PCA). 2,2-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 2, 2'-diphenyl-1-picrylhydrazyl radical (DPPH) radical scavenging assays were utilized for assessment of antioxidant activity, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dye reduction assay was used to determine cytotoxic activity against C-6 glioma rat brain, MCF-7 breast cancer, and HCT-15 colon cancer cell lines. The compounds were found to have significant antioxidant and cytotoxic activity. Since there is a considerable increase in characterizing novel chemical compounds from plant parts, the present study might be helpful for chemotaxonomic determinations, for understanding of medicinal properties as well as for the quality assessment of herbal supplements containing bark, thus establishing its use in traditional medicine.
Stigmasterol exerts antiglioma effects by regulating lipid metabolism.
Wei T, Li R, Guo S, Liang C Mol Med Rep. 2024; 30(6).
PMID: 39364731 PMC: 11484536. DOI: 10.3892/mmr.2024.13351.
Arafat S, Iqbal J, Abbasi B, Ijaz S, Yaseen T, Murtaza G Sci Rep. 2024; 14(1):21225.
PMID: 39261673 PMC: 11390931. DOI: 10.1038/s41598-024-72250-0.
Wang Y, Ai Q, Gu M, Guan H, Yang W, Zhang M Front Pharmacol. 2024; 15:1364948.
PMID: 38694910 PMC: 11061381. DOI: 10.3389/fphar.2024.1364948.
Pharmacological and Chemical Analysis of L. Using an In Vitro Antiadipogenic Model.
Islas-Garduno A, Romero-Cerecero O, Jimenez-Aparicio A, Tortoriello J, Montiel-Ruiz R, Gonzalez-Cortazar M Plants (Basel). 2023; 12(22).
PMID: 38005696 PMC: 10674420. DOI: 10.3390/plants12223799.
Liang C, Zhang B, Li R, Guo S, Fan X BMC Complement Med Ther. 2023; 23(1):342.
PMID: 37759283 PMC: 10523639. DOI: 10.1186/s12906-023-04174-7.