SOS Induction by Thermosensitive Replication Mutants of MiniF Plasmid

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1985 Jan 1

PMID 3159950

Citations 13

Authors

S Sommer

A Bailone

R Devoret

Affiliations

Soon will be listed here.

Abstract

MiniF, a 9.3 kb fragment of the dispensable F plasmid, carries genes necessary for its replication and partition as well as for the expression of an SOS signal. The arrest of replication of a thermo-sensitive miniFts at 42 degrees C induced SOS functions such as prophage lambda, sfiA expression, W-reactivation of UV-irradiated phage lambda. Two miniF ts9 and ts17 mutations were located within the KpnI fragment (43.6-46.9) in the minimal oriS replicon. Blocking miniF replication by incBC+ incompatibility genes situated in trans on a second plasmid also induced SOS functions. In contrast, if miniFts17 plasmid escaped the replication block at 42 degrees C by being inserted into pR325, there was no SOS induction. SOS induction by the arrest of miniF replication required the miniF lynA+ locus in cis, the host recA+ and lexA+ genes. We found that SOS induction was increased greatly near the stationary phase and that cell viability declined. During host cell exponential growth, miniFts9 and miniFts17 plasmids were lost rapidly, although SOS induction persisted for several cell generations. We postulate that lynA expresses a persistent product that may lead to the unwinding of chromosomal DNA.

Citing Articles

Expression and mutational analysis of DinB-like protein DR0053 in Deinococcus radiodurans.

Appukuttan D, Seo H, Jeong S, Im S, Joe M, Song D PLoS One. 2015; 10(2):e0118275.

PMID: 25706748 PMC: 4338110. DOI: 10.1371/journal.pone.0118275.

Tracking, tuning, and terminating microbial physiology using synthetic riboregulators.

Callura J, Dwyer D, Isaacs F, Cantor C, Collins J Proc Natl Acad Sci U S A. 2010; 107(36):15898-903.

PMID: 20713708 PMC: 2936621. DOI: 10.1073/pnas.1009747107.

DNA gyrase, topoisomerase IV, and the 4-quinolones.

Drlica K, Zhao X Microbiol Mol Biol Rev. 1997; 61(3):377-92.

PMID: 9293187 PMC: 232616. DOI: 10.1128/mmbr.61.3.377-392.1997.

An inhibitor of SOS induction, specified by a plasmid locus in Escherichia coli.

Bagdasarian M, Bailone A, Bagdasarian M, Manning P, Lurz R, Timmis K Proc Natl Acad Sci U S A. 1986; 83(15):5723-6.

PMID: 3526338 PMC: 386361. DOI: 10.1073/pnas.83.15.5723.

Mutational and in vivo methylation analysis of F-factor PifC protein binding to the pif operator and the region containing the primary origin of mini-F replication.

Miller J, MALAMY M Proc Natl Acad Sci U S A. 1986; 83(5):1433-7.

PMID: 3513175 PMC: 323090. DOI: 10.1073/pnas.83.5.1433.

References

Huisman O, Dari R . Effect of suppressors of SOS-mediated filamentation on sfiA operon expression in Escherichia coli. J Bacteriol. 1983; 153(1):169-75. PMC: 217354. DOI: 10.1128/jb.153.1.169-175.1983. View

Ogura T, Hiraga S . Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition. Cell. 1983; 32(2):351-60. DOI: 10.1016/0092-8674(83)90454-3. View

Quillardet P, Hofnung M . Induction by UV light of the SOS function sfiA in Escherichia coli strains deficient or proficient in excision repair. J Bacteriol. 1984; 157(1):35-8. PMC: 215125. DOI: 10.1128/jb.157.1.35-38.1984. View

Sogaard-Andersen L, Rokeach L, Molin S . Regulated expression of a gene important for replication of plasmid F in E. coli. EMBO J. 1984; 3(2):257-62. PMC: 557332. DOI: 10.1002/j.1460-2075.1984.tb01794.x. View

Seelke R, Kline B, Trawick J, Ritts G . Genetic studies of F plasmid maintenance genes involved in copy number control, incompatability, and partitioning. Plasmid. 1982; 7(2):163-79. DOI: 10.1016/0147-619x(82)90075-0. View

Little J, Edmiston S, Pacelli L, Mount D . Cleavage of the Escherichia coli lexA protein by the recA protease. Proc Natl Acad Sci U S A. 1980; 77(6):3225-9. PMC: 349587. DOI: 10.1073/pnas.77.6.3225. View

Little J, Mount D . The SOS regulatory system of Escherichia coli. Cell. 1982; 29(1):11-22. DOI: 10.1016/0092-8674(82)90085-x. View

George J, Devoret R . Conjugal transfer of UV-damaged F-prime sex factors and indirect induction of prophage- . Mol Gen Genet. 1971; 111(2):103-19. DOI: 10.1007/BF00267786. View

Craig N, Roberts J . Function of nucleoside triphosphate and polynucleotide in Escherichia coli recA protein-directed cleavage of phage lambda repressor. J Biol Chem. 1981; 256(15):8039-44. View

10.

Ebina Y, Takahara Y, Shirabe K, Yamada M, Nakazawa T, Nakazawa A . Plasmid-encoded regulation of colicin E1 gene expression. J Bacteriol. 1983; 156(2):487-92. PMC: 217858. DOI: 10.1128/jb.156.2.487-492.1983. View

11.

Cuzin F, Jacob F . [Mutations of the F episome of Escherichia coli K 12. II. Mutants with thermosensitive replication]. Ann Inst Pasteur (Paris). 1967; 112(4):397-418. View

12.

Moreau P, Fanica M, Devoret R . Induction of prophage lambda does not require full induction of RecA protein synthesis. Biochimie. 1980; 62(10):687-94. DOI: 10.1016/s0300-9084(80)80026-5. View

13.

Miki T, Yoshioka K, Horiuchi T . Control of cell division by sex factor F in Escherichia coli. I. The 42.84-43.6 F segment couples cell division of the host bacteria with replication of plasmid DNA. J Mol Biol. 1984; 174(4):605-25. DOI: 10.1016/0022-2836(84)90086-x. View

14.

Timmis K, Cabello F, Cohen S . Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A. 1975; 72(6):2242-6. PMC: 432733. DOI: 10.1073/pnas.72.6.2242. View

15.

Moreau P, Pelico J, Devoret R . Cleavage of lambda repressor and synthesis of RecA protein induced by transferred UV-damaged F sex factor. Mol Gen Genet. 1982; 186(2):170-9. DOI: 10.1007/BF00331847. View

16.

Gardner R, Malcolm L, Bergquist P, Lane H . IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group. Mol Gen Genet. 1982; 188(2):345-52. DOI: 10.1007/BF00332699. View

17.

George J, Devoret R, Radman M . Indirect ultraviolet-reactivation of phage lambda. Proc Natl Acad Sci U S A. 1974; 71(1):144-7. PMC: 387953. DOI: 10.1073/pnas.71.1.144. View

18.

Casaregola S, Dari R, Huisman O . Quantitative evaluation of recA gene expression in Escherichia coli. Mol Gen Genet. 1982; 185(3):430-9. DOI: 10.1007/BF00334135. View

19.

Karoui H, Bex F, Dreze P, Couturier M . Ham22, a mini-F mutation which is lethal to host cell and promotes recA-dependent induction of lambdoid prophage. EMBO J. 1983; 2(11):1863-8. PMC: 555381. DOI: 10.1002/j.1460-2075.1983.tb01672.x. View

20.

Gardner R, Caughey P, Lane D, Bergquist P . Replication mutants of the F-plasmid of Escherichia coli. II. Cloned replication regions of temperature-sensitive mutants. Plasmid. 1980; 3(2):179-92. DOI: 10.1016/0147-619x(80)90108-0. View