Structural and Functional Comparison Between the Stability Systems ParD of Plasmid R1 and Ccd of Plasmid F

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1991 Mar 1

PMID 2017133

Citations 7

Authors

G de Torrontegui

G Gimenez-Gallego

R Diaz-Orejas

Affiliations

Soon will be listed here.

Abstract

The stability determined by the systems ParD of plasmid R1 and Ccd of plasmid F is due to the concerted action of two proteins, a cytotoxin and an antagonist of this function. In this paper we report that CcdA and Kis proteins, the antagonists of the Ccd and ParD systems respectively, share significant sequence homologies at both ends. In Kis, these regions seem to correspond to two different domains. Despite the structural similarities, Kis and CcdA are not interchangeable. In addition we have shown that the cytotoxins of these systems, the Kid and CcdB proteins, do not share structural homologies. In contrast to CcdB, the Kid protein of the ParD system induces RecA-dependent cleavage of the cI repressor of bacteriophage lambda very inefficiently or not at all. The functional implications of these results are discussed.

Citing Articles

A common origin for the bacterial toxin-antitoxin systems parD and ccd, suggested by analyses of toxin/target and toxin/antitoxin interactions.

Smith A, Lopez-Villarejo J, Diago-Navarro E, Mitchenall L, Barendregt A, Heck A PLoS One. 2012; 7(9):e46499.

PMID: 23029540 PMC: 3460896. DOI: 10.1371/journal.pone.0046499.

The solution structure of ParD, the antidote of the ParDE toxin antitoxin module, provides the structural basis for DNA and toxin binding.

Oberer M, Zangger K, Gruber K, Keller W Protein Sci. 2007; 16(8):1676-88.

PMID: 17656583 PMC: 2203376. DOI: 10.1110/ps.062680707.

Modular organization of the Phd repressor/antitoxin protein.

Smith J, Magnuson R J Bacteriol. 2004; 186(9):2692-8.

PMID: 15090510 PMC: 387787. DOI: 10.1128/JB.186.9.2692-2698.2004.

The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins.

Oberer M, Zangger K, Prytulla S, Keller W Biochem J. 2001; 361(Pt 1):41-7.

PMID: 11743881 PMC: 1222296. DOI: 10.1042/0264-6021:3610041.

Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress.

Gerdes K J Bacteriol. 2000; 182(3):561-72.

PMID: 10633087 PMC: 94316. DOI: 10.1128/JB.182.3.561-572.2000.

References

Miki T, Yoshioka K, Horiuchi T . Control of cell division by sex factor F in Escherichia coli. I. The 42.84-43.6 F segment couples cell division of the host bacteria with replication of plasmid DNA. J Mol Biol. 1984; 174(4):605-25. DOI: 10.1016/0022-2836(84)90086-x. View

Hiraga S, Jaffe A, Ogura T, Mori H, Takahashi H . F plasmid ccd mechanism in Escherichia coli. J Bacteriol. 1986; 166(1):100-4. PMC: 214563. DOI: 10.1128/jb.166.1.100-104.1986. View

Sweet R, Eisenberg D . Correlation of sequence hydrophobicities measures similarity in three-dimensional protein structure. J Mol Biol. 1983; 171(4):479-88. DOI: 10.1016/0022-2836(83)90041-4. View

Rose G, Geselowitz A, Lesser G, Lee R, Zehfus M . Hydrophobicity of amino acid residues in globular proteins. Science. 1985; 229(4716):834-8. DOI: 10.1126/science.4023714. View

Brandenburger A, Bailone A, Levine A, Devoret R . Gratuitous induction. J Mol Biol. 1984; 179(3):571-6. DOI: 10.1016/0022-2836(84)90082-2. View

Gimenez-Gallego G, Navia M, Reuben J, KATZ G, Kaczorowski G, Garcia M . Purification, sequence, and model structure of charybdotoxin, a potent selective inhibitor of calcium-activated potassium channels. Proc Natl Acad Sci U S A. 1988; 85(10):3329-33. PMC: 280202. DOI: 10.1073/pnas.85.10.3329. View

APPLEYARD R . Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954; 39(4):440-52. PMC: 1209664. DOI: 10.1093/genetics/39.4.440. View

Tam J, Kline B . Control of the ccd operon in plasmid F. J Bacteriol. 1989; 171(5):2353-60. PMC: 209908. DOI: 10.1128/jb.171.5.2353-2360.1989. View

de Feyter R, Wallace C, Lane D . Autoregulation of the ccd operon in the F plasmid. Mol Gen Genet. 1989; 218(3):481-6. DOI: 10.1007/BF00332413. View

10.

Mori H, Ogura T, Hiraga S . Prophage lambda induction caused by mini-F plasmid genes. Mol Gen Genet. 1984; 196(2):185-93. DOI: 10.1007/BF00328049. View

11.

Tsuchimoto S, Ohtsubo H, Ohtsubo E . Two genes, pemK and pemI, responsible for stable maintenance of resistance plasmid R100. J Bacteriol. 1988; 170(4):1461-6. PMC: 210989. DOI: 10.1128/jb.170.4.1461-1466.1988. View

12.

Bravo A, Ortega S, de Torrontegui G, Diaz R . Killing of Escherichia coli cells modulated by components of the stability system ParD of plasmid R1. Mol Gen Genet. 1988; 215(1):146-51. DOI: 10.1007/BF00331316. View

13.

Yarmolinsky M, Stevens E . Replication-control functions block the induction of an SOS response by a damaged P1 bacteriophage. Mol Gen Genet. 1983; 192(1-2):140-8. DOI: 10.1007/BF00327659. View

14.

Karoui H, Bex F, Dreze P, Couturier M . Ham22, a mini-F mutation which is lethal to host cell and promotes recA-dependent induction of lambdoid prophage. EMBO J. 1983; 2(11):1863-8. PMC: 555381. DOI: 10.1002/j.1460-2075.1983.tb01672.x. View

15.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

16.

Bex F, Karoui H, Rokeach L, Dreze P, Garcia L, Couturier M . Mini-F encoded proteins: identification of a new 10.5 kilodalton species. EMBO J. 1983; 2(11):1853-61. PMC: 555380. DOI: 10.1002/j.1460-2075.1983.tb01671.x. View

17.

DONACHIE W, Begg K, Vicente M . Cell length, cell growth and cell division. Nature. 1976; 264(5584):328-33. DOI: 10.1038/264328a0. View

18.

Miki T, Orita T, Furuno M, Horiuchi T . Control of cell division by sex factor F in Escherichia coli. III. Participation of the groES (mopB) gene of the host bacteria. J Mol Biol. 1988; 201(2):327-38. DOI: 10.1016/0022-2836(88)90141-6. View

19.

Sommer S, Bailone A, Devoret R . SOS induction by thermosensitive replication mutants of miniF plasmid. Mol Gen Genet. 1985; 198(3):456-64. DOI: 10.1007/BF00332939. View

20.

Diaz R, Ortega S . Initiation of plasmid R1 replication in vitro is independent of transcription by host RNA polymerase. Nucleic Acids Res. 1984; 12(13):5175-91. PMC: 318912. DOI: 10.1093/nar/12.13.5175. View