Rv0100, a Proposed Acyl Carrier Protein in Mycobacterium Tuberculosis: Expression, Purification and Crystallization

Overview

Journal Acta Crystallogr F Struct Biol Commun

Specialty Chemistry

Date 2019 Oct 5

PMID 31584013

Citations 2

Authors

Jasper Marc G Bondoc

Hiten J Gutka

Mashal M Almutairi

Ryan Patwell

Maxwell W Rutter

Nina M Wolf

Ram Samudrala

Shahila Mehboob

Farahnaz Movahedzadeh

Affiliations

Soon will be listed here.

Abstract

Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 Å. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.

Citing Articles

Rv0100: An essential acyl carrier protein from M. tuberculosis important in dormancy.

Gutka H, Bondoc J, Patwell R, Khan S, Grzelak E, Goswami R PLoS One. 2024; 19(6):e0304876.

PMID: 38848336 PMC: 11161019. DOI: 10.1371/journal.pone.0304876.

Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: expression, purification and crystallization. Corrigendum.

Bondoc J, Gutka H, Almutairi M, Patwell R, Rutter M, Wolf N Acta Crystallogr F Struct Biol Commun. 2020; 76(Pt 4):192-193.

PMID: 32254053 PMC: 7137385. DOI: 10.1107/S2053230X2000271X.

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