Developmental Changes of RRNA Ribose Methylations in the Mouse

Overview

Journal RNA Biol

Specialty Molecular Biology

Date 2019 Oct 1

PMID 31566069

Citations 35

Authors

Jade Hebras

Nicolai Krogh

Virginie Marty

Henrik Nielsen

Jerome Cavaille

Affiliations

Soon will be listed here.

Abstract

A sequencing-based profiling method (RiboMeth-seq) for ribose methylations was used to study methylation patterns in mouse adult tissues and during development. In contrast to previous reports based on studies of human cancer cell lines, almost all methylation sites were close to fully methylated in adult tissues. A subset of sites was differentially modified in developing tissues compared to their adult counterparts and showed clear developmental dynamics. This provides the first evidence for ribosome heterogeneity at the level of rRNA modifications during mouse development. In a prominent example, the expression levels of SNORD78 during development appeared to be regulated by alternative splicing of the host-gene and to correlate with the methylation level of its target site at LSU-G4593. The results are discussed in the context of the specialized ribosome hypothesis.

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