DREADDs: The Power of the Lock, the Weakness of the Key. Favoring the Pursuit of Specific Conditions Rather Than Specific Ligands

Overview

Journal eNeuro

Specialty Neurology

Date 2019 Sep 29

PMID 31562177

Citations 18

Authors

Raphael Goutaudier

Veronique Coizet

Carole Carcenac

Sebastien Carnicella

Affiliations

Soon will be listed here.

Citing Articles

Unveiling the brain's symphony: exploring the necessity and sufficiency of neural networks in behavior control.

Bustos F Neural Regen Res. 2024; 20(1):186-187.

PMID: 39657083 PMC: 11246130. DOI: 10.4103/NRR.NRR-D-23-02084.

Impacts of sex differences on optogenetic, chemogenetic, and calcium-imaging tools.

Cea Salazar V, Perez M, Robison A, Trainor B Curr Opin Neurobiol. 2023; 84:102817.

PMID: 38042130 PMC: 11374099. DOI: 10.1016/j.conb.2023.102817.

Chemogenetic approaches reveal dual functions of microglia in seizures.

Dheer A, Bosco D, Zheng J, Wang L, Zhao S, Haruwaka K Brain Behav Immun. 2023; 115:406-418.

PMID: 37926132 PMC: 10841657. DOI: 10.1016/j.bbi.2023.11.002.

Clozapine N-oxide, compound 21, and JHU37160 do not influence effortful reward-seeking behavior in mice.

Aomine Y, Oyama Y, Sakurai K, Macpherson T, Ozawa T, Hikida T Psychopharmacology (Berl). 2023; 241(1):89-96.

PMID: 37792024 PMC: 10774210. DOI: 10.1007/s00213-023-06465-w.

Comparison of three DREADD agonists acting on Gq-DREADDs in the ventral tegmental area to alter locomotor activity in tyrosine hydroxylase:Cre male and female rats.

Robinson H, Nicholson K, Shelton K, Hamilton P, Banks M Behav Brain Res. 2023; 455:114674.

PMID: 37722510 PMC: 10918529. DOI: 10.1016/j.bbr.2023.114674.

References

Wiegert J, Mahn M, Prigge M, Printz Y, Yizhar O . Silencing Neurons: Tools, Applications, and Experimental Constraints. Neuron. 2017; 95(3):504-529. PMC: 5830081. DOI: 10.1016/j.neuron.2017.06.050. View

SCHOTTE A, Janssen P, Megens A, Leysen J . Occupancy of central neurotransmitter receptors by risperidone, clozapine and haloperidol, measured ex vivo by quantitative autoradiography. Brain Res. 1993; 631(2):191-202. DOI: 10.1016/0006-8993(93)91535-z. View

Chen X, Choo H, Huang X, Yang X, Stone O, Roth B . The first structure-activity relationship studies for designer receptors exclusively activated by designer drugs. ACS Chem Neurosci. 2015; 6(3):476-84. PMC: 4368042. DOI: 10.1021/cn500325v. View

Mahler S, Vazey E, Beckley J, Keistler C, McGlinchey E, Kaufling J . Designer receptors show role for ventral pallidum input to ventral tegmental area in cocaine seeking. Nat Neurosci. 2014; 17(4):577-85. PMC: 3973180. DOI: 10.1038/nn.3664. View

Owen S, Liu M, Kreitzer A . Thermal constraints on in vivo optogenetic manipulations. Nat Neurosci. 2019; 22(7):1061-1065. PMC: 6592769. DOI: 10.1038/s41593-019-0422-3. View

Mahler S, Aston-Jones G . CNO Evil? Considerations for the Use of DREADDs in Behavioral Neuroscience. Neuropsychopharmacology. 2018; 43(5):934-936. PMC: 5854815. DOI: 10.1038/npp.2017.299. View

Baerentzen S, Casado-Sainz A, Lange D, Shalgunov V, Martinez Tejada I, Xiong M . The Chemogenetic Receptor Ligand Clozapine N-Oxide Induces Neuroreceptor Occupancy and Reduces Striatal Glutamate Levels. Front Neurosci. 2019; 13:187. PMC: 6456655. DOI: 10.3389/fnins.2019.00187. View

Aldrin-Kirk P, Bjorklund T . Practical Considerations for the Use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain. Methods Mol Biol. 2019; 1937:59-87. DOI: 10.1007/978-1-4939-9065-8_4. View

Vardy E, Robinson J, Li C, Olsen R, DiBerto J, Giguere P . A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior. Neuron. 2015; 86(4):936-946. PMC: 4441592. DOI: 10.1016/j.neuron.2015.03.065. View

10.

Koob G, Schulkin J . Addiction and stress: An allostatic view. Neurosci Biobehav Rev. 2018; 106:245-262. DOI: 10.1016/j.neubiorev.2018.09.008. View

11.

Ilg A, Enkel T, Bartsch D, Bahner F . Behavioral Effects of Acute Systemic Low-Dose Clozapine in Wild-Type Rats: Implications for the Use of DREADDs in Behavioral Neuroscience. Front Behav Neurosci. 2018; 12:173. PMC: 6102325. DOI: 10.3389/fnbeh.2018.00173. View

12.

Campbell E, Marchant N . The use of chemogenetics in behavioural neuroscience: receptor variants, targeting approaches and caveats. Br J Pharmacol. 2018; 175(7):994-1003. PMC: 5843707. DOI: 10.1111/bph.14146. View

13.

Sternson S, Roth B . Chemogenetic tools to interrogate brain functions. Annu Rev Neurosci. 2014; 37:387-407. DOI: 10.1146/annurev-neuro-071013-014048. View

14.

Sousa N, Almeida O, Wotjak C . A hitchhiker's guide to behavioral analysis in laboratory rodents. Genes Brain Behav. 2006; 5 Suppl 2:5-24. DOI: 10.1111/j.1601-183X.2006.00228.x. View

15.

Boekhoudt L, Omrani A, Luijendijk M, Wolterink-Donselaar I, Wijbrans E, van der Plasse G . Chemogenetic activation of dopamine neurons in the ventral tegmental area, but not substantia nigra, induces hyperactivity in rats. Eur Neuropsychopharmacol. 2016; 26(11):1784-1793. DOI: 10.1016/j.euroneuro.2016.09.003. View

16.

Sorge R, Martin L, Isbester K, Sotocinal S, Rosen S, Tuttle A . Olfactory exposure to males, including men, causes stress and related analgesia in rodents. Nat Methods. 2014; 11(6):629-32. DOI: 10.1038/nmeth.2935. View

17.

Whissell P, Tohyama S, Martin L . The Use of DREADDs to Deconstruct Behavior. Front Genet. 2016; 7:70. PMC: 4868840. DOI: 10.3389/fgene.2016.00070. View

18.

Saloman J, Scheff N, Snyder L, Ross S, Davis B, Gold M . Gi-DREADD Expression in Peripheral Nerves Produces Ligand-Dependent Analgesia, as well as Ligand-Independent Functional Changes in Sensory Neurons. J Neurosci. 2016; 36(42):10769-10781. PMC: 5083007. DOI: 10.1523/JNEUROSCI.3480-15.2016. View

19.

Gomez J, Bonaventura J, Lesniak W, Mathews W, Sysa-Shah P, Rodriguez L . Chemogenetics revealed: DREADD occupancy and activation via converted clozapine. Science. 2017; 357(6350):503-507. PMC: 7309169. DOI: 10.1126/science.aan2475. View

20.

Magnus C, Lee P, Atasoy D, Su H, Looger L, Sternson S . Chemical and genetic engineering of selective ion channel-ligand interactions. Science. 2011; 333(6047):1292-6. PMC: 3210548. DOI: 10.1126/science.1206606. View