Gi-DREADD Expression in Peripheral Nerves Produces Ligand-Dependent Analgesia, As Well As Ligand-Independent Functional Changes in Sensory Neurons

Overview

Journal J Neurosci

Specialty Neurology

Date 2016 Nov 1

PMID 27798132

Citations 53

Authors

Jami L Saloman

Nicole N Scheff

Lindsey M Snyder

Sarah E Ross

Brian M Davis

Michael S Gold

Affiliations

Soon will be listed here.

Abstract

Significance Statement: DREADD technology is a powerful tool enabling manipulation of activity and/or transmitter release from targeted cell populations. The purpose of this study was to determine whether inhibitory DREADDs in nociceptive afferents could be used to produce analgesia, and if so, how. DREADD activation produced a ligand-dependent analgesia to heat in vivo and a decrease in neuronal firing at the single-cell level. However, we observed that expression of Gi-DREADD also causes ligand-independent changes in ion channel activity and second-messenger signaling. These findings highlight both the potential and the limitations of this exciting technology as well as the necessity to identify the mechanisms underlying any observed phenotype.

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