INPP1 Up-regulation by MiR-27a Contributes to the Growth, Migration and Invasion of Human Cervical Cancer

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2019 Sep 27

PMID 31557403

Citations 7

Authors

Pu Li

Qiaoge Zhang

Hua Tang

Affiliations

Soon will be listed here.

Abstract

Inositol polyphosphate-1-phosphatase (INPP1) is an enzyme that is responsible for glycolysis and lipid metabolism. Here, we discovered that INPP1 expression was up-regulated in CC tissues compared to that in adjacent normal tissues by RT-qPCR. Inositol polyphosphate-1-phosphatase overexpression promoted and INPP1 knockdown suppressed cell viability, cellular migration/invasion and EMT in CC cells. To explore the mechanism of dysregulation, INPP1 was predicted to be a target of miR-27a, and a pmiRGLO dual-luciferase reporter assay showed that miR-27a bound to the 3' UTR of INPP1. RT-qPCR revealed that miR-27a was also up-regulated and had a positive correlation with INPP1 expression in CC tissues. Furthermore, shR-INPP1 could favour the malignant phenotype reversion induced by miR-27a, suggesting that miR-27a up-regulates INPP1 to promote tumorigenic activities. Altogether, our findings show that the up-regulation of INPP1 by miR-27a contributes to tumorigenic activities and may provide a potential biomarker for CC.

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References

Gocze K, Gombos K, Kovacs K, Juhasz K, Gocze P, Kiss I . MicroRNA expressions in HPV-induced cervical dysplasia and cancer. Anticancer Res. 2015; 35(1):523-30. View

Song G, Wang R, Guo J, Liu X, Wang F, Qi Y . miR-346 and miR-138 competitively regulate hTERT in GRSF1- and AGO2-dependent manners, respectively. Sci Rep. 2015; 5:15793. PMC: 4623477. DOI: 10.1038/srep15793. View

Long M, Wu F, Li P, Liu M, Li X, Tang H . MicroRNA-10a targets CHL1 and promotes cell growth, migration and invasion in human cervical cancer cells. Cancer Lett. 2012; 324(2):186-96. DOI: 10.1016/j.canlet.2012.05.022. View

Luo W, Zhang D, Ma S, Wang C, Zhang Q, Wang H . miR-27a is highly expressed in H1650 cancer stem cells and regulates proliferation, migration, and invasion. J Cancer Res Ther. 2018; 14(Supplement):S1004-S1011. DOI: 10.4103/0973-1482.199450. View

Zhang L, Liu M, Li X, Tang H . miR-490-3p modulates cell growth and epithelial to mesenchymal transition of hepatocellular carcinoma cells by targeting endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3). J Biol Chem. 2012; 288(6):4035-47. PMC: 3567655. DOI: 10.1074/jbc.M112.410506. View

Granados Lopez A, Lopez J . Multistep model of cervical cancer: participation of miRNAs and coding genes. Int J Mol Sci. 2014; 15(9):15700-33. PMC: 4200848. DOI: 10.3390/ijms150915700. View

Lu J, Getz G, Miska E, Alvarez-Saavedra E, Lamb J, Peck D . MicroRNA expression profiles classify human cancers. Nature. 2005; 435(7043):834-8. DOI: 10.1038/nature03702. View

Li W, Yu Z, Ma B . The increase of miR-27a affects the role of cisplatin on proliferation and migration capacities of liver cancer cells. Eur Rev Med Pharmacol Sci. 2018; 22(17):5490-5498. DOI: 10.26355/eurrev_201809_15809. View

Torre L, Bray F, Siegel R, Ferlay J, Lortet-Tieulent J, Jemal A . Global cancer statistics, 2012. CA Cancer J Clin. 2015; 65(2):87-108. DOI: 10.3322/caac.21262. View

10.

Li Z, Rana T . Therapeutic targeting of microRNAs: current status and future challenges. Nat Rev Drug Discov. 2014; 13(8):622-38. DOI: 10.1038/nrd4359. View

11.

Amare A, Schubert K, Baune B . Pharmacogenomics in the treatment of mood disorders: Strategies and Opportunities for personalized psychiatry. EPMA J. 2017; 8(3):211-227. PMC: 5607053. DOI: 10.1007/s13167-017-0112-8. View

12.

Pardini B, De Maria D, Francavilla A, Di Gaetano C, Ronco G, Naccarati A . MicroRNAs as markers of progression in cervical cancer: a systematic review. BMC Cancer. 2018; 18(1):696. PMC: 6020348. DOI: 10.1186/s12885-018-4590-4. View

13.

Kumar L, Harish P, Malik P, Khurana S . Chemotherapy and targeted therapy in the management of cervical cancer. Curr Probl Cancer. 2018; 42(2):120-128. DOI: 10.1016/j.currproblcancer.2018.01.016. View

14.

Sun Y, Yang X, Liu M, Tang H . B4GALT3 up-regulation by miR-27a contributes to the oncogenic activity in human cervical cancer cells. Cancer Lett. 2016; 375(2):284-292. DOI: 10.1016/j.canlet.2016.03.016. View

15.

Benjamin D, Louie S, Mulvihill M, Kohnz R, Li D, Chan L . Inositol phosphate recycling regulates glycolytic and lipid metabolism that drives cancer aggressiveness. ACS Chem Biol. 2014; 9(6):1340-50. PMC: 4076040. DOI: 10.1021/cb5001907. View

16.

Del Mar Diaz-Gonzalez S, Deas J, Benitez-Boijseauneau O, Gomez-Ceron C, Bermudez-Morales V, Rodriguez-Dorantes M . Utility of microRNAs and siRNAs in cervical carcinogenesis. Biomed Res Int. 2015; 2015:374924. PMC: 4385600. DOI: 10.1155/2015/374924. View

17.

Kumar M, Lu J, Mercer K, Golub T, Jacks T . Impaired microRNA processing enhances cellular transformation and tumorigenesis. Nat Genet. 2007; 39(5):673-7. DOI: 10.1038/ng2003. View

18.

Wan H, Li Q, Zhang Y, Tian W, Li Y, Liu M . MiR-124 represses vasculogenic mimicry and cell motility by targeting amotL1 in cervical cancer cells. Cancer Lett. 2014; 355(1):148-58. DOI: 10.1016/j.canlet.2014.09.005. View

19.

Wang X, Wang H, Li Y, Hafner M, Banerjee N, Tang S . microRNAs are biomarkers of oncogenic human papillomavirus infections. Proc Natl Acad Sci U S A. 2014; 111(11):4262-7. PMC: 3964092. DOI: 10.1073/pnas.1401430111. View

20.

Guo J, Lv J, Liu M, Tang H . miR-346 Up-regulates Argonaute 2 (AGO2) Protein Expression to Augment the Activity of Other MicroRNAs (miRNAs) and Contributes to Cervical Cancer Cell Malignancy. J Biol Chem. 2015; 290(51):30342-50. PMC: 4683258. DOI: 10.1074/jbc.M115.691857. View