Development of Fluorescent Reagent Based on Ligand Exchange Reaction for the Highly Sensitive and Selective Detection of Dopamine in the Serum

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2019 Sep 25

PMID 31547244

Citations 3

Authors

Yoshio Suzuki

Affiliations

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Abstract

A new fluorescent probe () was developed for targeting dopamine, with a boron-dipyrromethenyl (BDP) group as the fluorophore and a Fe complex as the ligand exchange site. The free form of in solution displayed weak fluorescence emission, while it showed strong fluorescence emission after interaction with dopamine due to the release of Fe from , confirming the binding of Fe to dopamine. The increase in fluorescence intensity was concentration-dependent, and a good linear relationship was observed between the fluorescence intensity and dopamine concentration. The detection limit of dopamine by was 1.1 nM, indicating a 20-fold higher sensitivity than that of previously reported compounds. The reaction of with dopamine was not affected by the presence of foreign substances, allowing the highly selective detection of dopamine in the human serum sample. The results of this study indicate that the novel compound is a reliable fluorescent molecular probe for the detection of dopamine and can be widely employed in diverse scientific areas.

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