Detection of in Common Marmoset () and Treatment with Metronidazole

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2019 Sep 24

PMID 31543923

Citations 3

Authors

Eui-Suk Jeong

Jong-Hyung Park

Seung-Hyun Ryu

Soo-Young Choi

Kyoung-Sun Lee

Jong-Man Kim

Byung-Hwa Hyun

Yang-Kyu Choi

Affiliations

Soon will be listed here.

Abstract

Background: Recently, the use of common marmoset () has increased in biomedical research as an animal model. This study aimed to test fecal samples to monitor bacterial and parasite infections in common marmoset at the Laboratory Animal Center of Osong Medical Innovation Foundation in Korea.

Methods: To monitor bacteria and parasites in common marmoset, we tested 43 fecal samples of 43 common marmosets by culture and parasitological test in 2014. Infection by was determined by PCR method.

Results: We identified nonpathogenic bacteria such as and in feces of normal common marmosets. Interestingly, was isolated from a healthy common marmoset by fecal centrifugation concentration and PCR. The monkey infected with was treated with metronidazole. After the treatment, were not found in feces using fecal centrifugation concentration and PCR.

Conclusion: This is the first case report of infection in common marmoset. Treatment with metronidazole is found to be highly effective in eradicating infection in common marmoset.

Citing Articles

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Clinical Management of Gastrointestinal Disease in the Common Marmoset (Callithrix jacchus).

Fitz C, Goodroe A, Wierenga L, Mejia A, Simmons H ILAR J. 2021; 61(2-3):199-217.

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References

. Health monitoring of non-human primate colonies. Recommendations of the Federation of European Laboratory Animal Science Associations (FELASA) Working Group on non-human primate health accepted by the FELASA Board of Management, 21 November 1998. Lab Anim. 2000; 33 Suppl 1:S1-18. View

Scorza A, Lappin M . Metronidazole for the treatment of feline giardiasis. J Feline Med Surg. 2004; 6(3):157-60. PMC: 10822345. DOI: 10.1016/j.jfms.2003.11.007. View

Levecke B, Dorny P, Geurden T, Vercammen F, Vercruysse J . Gastrointestinal protozoa in non-human primates of four zoological gardens in Belgium. Vet Parasitol. 2007; 148(3-4):236-46. DOI: 10.1016/j.vetpar.2007.06.020. View

Nicklas W . International harmonization of health monitoring. ILAR J. 2008; 49(3):338-46. DOI: 10.1093/ilar.49.3.338. View

Youn H . Review of zoonotic parasites in medical and veterinary fields in the Republic of Korea. Korean J Parasitol. 2009; 47 Suppl:S133-41. PMC: 2769215. DOI: 10.3347/kjp.2009.47.S.S133. View

Parkinson C, OBrien A, Albers T, Simon M, Clifford C, Pritchett-Corning K . Diagnosis of ecto- and endoparasites in laboratory rats and mice. J Vis Exp. 2011; (55):e2767. PMC: 3230210. DOI: 10.3791/2767. View

Morimoto N, Korenaga M, Nishida Y, Takeuchi H, Kumon Y, Sugiura T . PCR amplification and DNA sequence analysis of parasitic intestinal protozoa in specimens stained with Chlorazol Black E. Acta Parasitol. 2013; 58(2):132-8. DOI: 10.2478/s11686-013-0119-9. View

Labberton L, Bakker J, Klomp R, Langermans J, van Geijlswijk I . Challenges in oral administration of metronidazole dissolved in drinking water to rhesus monkeys (Macaca mulatta). Lab Anim (NY). 2013; 42(6):213-6. DOI: 10.1038/laban.264. View

Mahler Convenor M, Berard M, Feinstein R, Gallagher A, Illgen-Wilcke B, Pritchett-Corning K . FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units. Lab Anim. 2014; 48(3):178-192. DOI: 10.1177/0023677213516312. View

10.

Drakulovski P, Bertout S, Locatelli S, Butel C, Pion S, Mpoudi-Ngole E . Assessment of gastrointestinal parasites in wild chimpanzees (Pan troglodytes troglodytes) in southeast Cameroon. Parasitol Res. 2014; 113(7):2541-50. PMC: 4245021. DOI: 10.1007/s00436-014-3904-y. View

11.

Schultz E, Haenni M, Mereghetti L, Siebor E, Neuwirth C, Madec J . Survey of multidrug resistance integrative mobilizable elements SGI1 and PGI1 in Proteus mirabilis in humans and dogs in France, 2010-13. J Antimicrob Chemother. 2015; 70(9):2543-6. DOI: 10.1093/jac/dkv154. View

12.

Inoue T, Hayashimoto N, Yasuda M, Sasaki E, Itoh T . Pentatrichomonas hominis in laboratory-bred common marmosets. Exp Anim. 2015; 64(4):363-8. PMC: 4637372. DOI: 10.1538/expanim.15-0010. View

13.

Ross C, Austad S, Brasky K, Brown C, Forney L, Gelfond J . The development of a specific pathogen free (SPF) barrier colony of marmosets () for aging research. Aging (Albany NY). 2017; 9(12):2544-2558. PMC: 5764392. DOI: 10.18632/aging.101340. View

14.

Riley L, Franklin C, Hook Jr R, Besch-Williford C . Identification of murine helicobacters by PCR and restriction enzyme analyses. J Clin Microbiol. 1996; 34(4):942-6. PMC: 228922. DOI: 10.1128/jcm.34.4.942-946.1996. View