A Comparative Evaluation of Regulatory T Cells Profile Among Acute and Chronic Cutaneous Leishmaniasis Using Flow Cytometry

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2019 Sep 24

PMID 31543906

Citations 3

Authors

Fahimeh Firouzjaie-Karder

Behnaz Akhoundi

Mehdi Mohebali

Farid Azmoudeh Ardalan

Abbas Rahimi-Foroushani

Fatemeh Mesgarian

Homa Hajjaran

Hossein Mortazavi

Yadollah Shakiba

Sorour Charehdar

Samira Elikaee

Zahra Kakooei

Zahra Shafeghat

Affiliations

Soon will be listed here.

Abstract

Background: Cutaneous leishmaniasis (CL) is described as a major health problem in many countries of the world. Regulatory T cells (Tregs) are characterized as one of immunologic indexes. One of the best methods to determine of Tregs percentage is flow cytometry. The aim of this study was determination of the role of Tregs profile among acute and chronic forms of human CL using flow cytometry analysis.

Methods: This study was conducted on 24 patients referred to Laboratory of Leishmaniasis, Tehran University of Medical Sciences, Tehran, Iran with acute and 14 patients with chronic phases of CL as well as 15 healthy individuals as control group in 2015-2016. After microscopic examination, 2 ml of peripheral blood samples were collected for determining percentage of CD CD CD low Tregs by using flow cytometry method.

Results: Using flow cytometry analysis, the average percentage of Tregs were calculated 5.73, 6.71 and 6.61 for acute, chronic and healthy individuals, respectively. With SPSS software and Scheffe multiple comparison tests, the differences within in these groups are statistically significant (=0.04) and between the acute and chronic group, there was marginally significant with approximately 91% of confidence level (=0.088).

Conclusion: Marginally differences were found significantly among averages of Regulatory T cells, acute and chronic phases of CL. Further comprehensive studies can be needed to verify the role of Tregs in both phases of CL cases.

Citing Articles

Immuno-Informatics Insight into the Relationship Between Cholesterol and Cytokines in Cutaneous Leishmaniasis: From clinics to computation.

Sulaiman E, Mohammad L, Thanoon A, Karimi I Sultan Qaboos Univ Med J. 2024; 24(4):507-514.

PMID: 39634810 PMC: 11614011. DOI: 10.18295/squmj.7.2024.043.

The level of interleukin-17, 23, and gamma interferon in cutaneous leishmaniasis patients before and after intra lesion treatment.

Ghazanfari M, Shahriari B, Rahnama V, Khazaei M, Naderi S, Motazedian M J Parasit Dis. 2022; 46(2):476-482.

PMID: 35692466 PMC: 9177927. DOI: 10.1007/s12639-021-01428-4.

The Comparison of the IFN-ɤ, TNF-α and IL-10 Cytokines in Healing and Non-healing Cutaneous Leishmaniasis.

Taraghian M, Hanif H, Mousavi P, Cheshmeh Z, Samei A, Abdollahi A Iran J Parasitol. 2021; 16(3):490-498.

PMID: 34630595 PMC: 8476719. DOI: 10.18502/ijpa.v16i3.7103.

References

Gaafar A, Veress B, Permin H, Kharazmi A, Theander T, El Hassan A . Characterization of the local and systemic immune responses in patients with cutaneous leishmaniasis due to Leishmania major. Clin Immunol. 1999; 91(3):314-20. DOI: 10.1006/clim.1999.4705. View

Bogdan C, Donhauser N, Doring R, Rollinghoff M, Diefenbach A, Rittig M . Fibroblasts as host cells in latent leishmaniosis. J Exp Med. 2000; 191(12):2121-30. PMC: 2193203. DOI: 10.1084/jem.191.12.2121. View

Belkaid Y, Hoffmann K, Mendez S, Kamhawi S, Udey M, Wynn T . The role of interleukin (IL)-10 in the persistence of Leishmania major in the skin after healing and the therapeutic potential of anti-IL-10 receptor antibody for sterile cure. J Exp Med. 2001; 194(10):1497-506. PMC: 2193677. DOI: 10.1084/jem.194.10.1497. View

Da-Cruz A, Bittar R, Mattos M, Nogueira R, Pinho-Ribeiro V, Azeredo-Coutinho R . T-cell-mediated immune responses in patients with cutaneous or mucosal leishmaniasis: long-term evaluation after therapy. Clin Diagn Lab Immunol. 2002; 9(2):251-6. PMC: 119941. DOI: 10.1128/cdli.9.2.251-256.2002. View

Hepburn N . Cutaneous leishmaniasis: an overview. J Postgrad Med. 2003; 49(1):50-4. DOI: 10.4103/0022-3859.928. View

Chen W, Jin W, Hardegen N, Lei K, Li L, Marinos N . Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3. J Exp Med. 2003; 198(12):1875-86. PMC: 2194145. DOI: 10.1084/jem.20030152. View

Mendez S, Reckling S, Piccirillo C, Sacks D, Belkaid Y . Role for CD4(+) CD25(+) regulatory T cells in reactivation of persistent leishmaniasis and control of concomitant immunity. J Exp Med. 2004; 200(2):201-10. PMC: 2212012. DOI: 10.1084/jem.20040298. View

Rouse B, Suvas S . Regulatory cells and infectious agents: detentes cordiale and contraire. J Immunol. 2004; 173(4):2211-5. DOI: 10.4049/jimmunol.173.4.2211. View

Suffia I, Reckling S, Salay G, Belkaid Y . A role for CD103 in the retention of CD4+CD25+ Treg and control of Leishmania major infection. J Immunol. 2005; 174(9):5444-55. DOI: 10.4049/jimmunol.174.9.5444. View

10.

Ji J, Masterson J, Sun J, Soong L . CD4+CD25+ regulatory T cells restrain pathogenic responses during Leishmania amazonensis infection. J Immunol. 2005; 174(11):7147-53. PMC: 2812412. DOI: 10.4049/jimmunol.174.11.7147. View

11.

Campanelli A, Roselino A, Cavassani K, Pereira M, Mortara R, Brodskyn C . CD4+CD25+ T cells in skin lesions of patients with cutaneous leishmaniasis exhibit phenotypic and functional characteristics of natural regulatory T cells. J Infect Dis. 2006; 193(9):1313-22. DOI: 10.1086/502980. View

12.

Ajdary S, Jafari-Shakib R, Riazi-Rad F, Khamesipour A . Soluble CD26 and CD30 levels in patients with anthroponotic cutaneous leishmaniasis. J Infect. 2007; 55(1):75-8. DOI: 10.1016/j.jinf.2006.12.005. View

13.

Bacchetta R, Gambineri E, Roncarolo M . Role of regulatory T cells and FOXP3 in human diseases. J Allergy Clin Immunol. 2007; 120(2):227-35. DOI: 10.1016/j.jaci.2007.06.023. View

14.

Nagase H, Jones K, Anderson C, Noben-Trauth N . Despite increased CD4+Foxp3+ cells within the infection site, BALB/c IL-4 receptor-deficient mice reveal CD4+Foxp3-negative T cells as a source of IL-10 in Leishmania major susceptibility. J Immunol. 2007; 179(4):2435-44. DOI: 10.4049/jimmunol.179.4.2435. View

15.

Bittencourt A, Barral A . Evaluation of the histopathological classifications of American cutaneous and mucocutaneous leishmaniasis. Mem Inst Oswaldo Cruz. 1991; 86(1):51-6. DOI: 10.1590/s0074-02761991000100009. View

16.

Jafari-Shakib R, Shokrgozar M, Nassiri-Kashani M, Malakafzali B, Nikbin B, Khamesipour A . Plasma sCD26 and sCD30 levels in cutaneous leishmaniasis. Acta Trop. 2008; 109(1):61-3. DOI: 10.1016/j.actatropica.2008.09.018. View

17.

Rodrigues O, Marques C, Soares-Clemente M, Ferronha M, Santos-Gomes G . Identification of regulatory T cells during experimental Leishmania infantum infection. Immunobiology. 2009; 214(2):101-11. DOI: 10.1016/j.imbio.2008.07.001. View

18.

Maurya R, Kumar R, Prajapati V, Manandhar K, Sacks D, Sundar S . Human visceral leishmaniasis is not associated with expansion or accumulation of Foxp3+ CD4 cells in blood or spleen. Parasite Immunol. 2010; 32(7):479-83. PMC: 2898731. DOI: 10.1111/j.1365-3024.2010.01219.x. View

19.

Gupta G, Majumdar S, Adhikari A, Bhattacharya P, Mukherjee A, Bhattacharyya Majumdar S . Treatment with IP-10 induces host-protective immune response by regulating the T regulatory cell functioning in Leishmania donovani-infected mice. Med Microbiol Immunol. 2011; 200(4):241-53. DOI: 10.1007/s00430-011-0197-y. View

20.

Rai A, Thakur C, Singh A, Seth T, Srivastava S, Singh P . Regulatory T cells suppress T cell activation at the pathologic site of human visceral leishmaniasis. PLoS One. 2012; 7(2):e31551. PMC: 3275558. DOI: 10.1371/journal.pone.0031551. View