Histology of Skeletal Muscle Reconstructed by Means of the Implantation of Autologous Adipose Tissue: an Experimental Study

Overview

Journal Histol Histopathol

Specialties Anatomy & Histology
Pathology

Date 2019 Sep 17

PMID 31523800

Citations 3

Authors

Fernando Leiva-Cepas

Ignacio Jimena

Ignacio Ruz-Caracuel

Evelio Luque

Rafael Villalba

Jose Pena-Amaro

Affiliations

Soon will be listed here.

Abstract

The purpose of this study was to determine the histological characteristics of a skeletal muscle reconstructed by means of the implantation of autologous adipose tissue following an experimentally-induced volumetric muscle loss. A cylindrical piece in the belly of the rat anterior tibial muscle was removed. In the hole, inguinal subcutaneous adipose tissue of the same rat was grafted. Animals were sacrificed 7, 14, 21, 28 and 60 days posttransplantation. Histological, histochemical, immunohistochemical and morphometric techniques were used. At all times analyzed, the regenerative muscle fibers formed from the edges of the muscle tissue showed histological, histochemical and immunohistochemical differences in comparison with the control group. These differences are related to delays in the maturation process and are related to problems in reinnervation and disorientation of muscle fibers. The stains for MyoD and desmin showed that some myoblasts and myotubes seem to derive from the transplanted adipose tissue. After 60 days, the transplant area was 20% occupied by fibrosis and by 80% skeletal muscle. However, the neo-muscle was chaotically organized showing muscle fiber disorientation and centronucleated fibers with irregular shape and size. Our results support the hypothesis that, at least from a morphological point of view, autologous adipose tissue transplantation favors reconstruction following a volumetric loss of skeletal muscle by combining the inherent regenerative response of the organ itself and the myogenic differentiation of the stem cells present in the adipose tissue. However, in our study, the formed neo-muscle exhibited histological differences in comparison with the normal skeletal muscle.

Citing Articles

Influence of Physical Exercise on the Rehabilitation of Volumetric Muscle Loss Injury Reconstructed with Autologous Adipose Tissue.

Lopez-Espejo M, Jimena I, Gil-Belmonte M, Rivero J, Pena-Amaro J J Funct Morphol Kinesiol. 2024; 9(4).

PMID: 39449482 PMC: 11503405. DOI: 10.3390/jfmk9040188.

Acute and chronic mammary periprosthetic histological changes of the muscle.

Camara-Perez J, Jimena I, Rodriguez-Cano M, Sanz-Zorrilla A, Osuna-Soto J, Sanchez-Ramirez I JPRAS Open. 2024; 41:265-275.

PMID: 39170095 PMC: 11338051. DOI: 10.1016/j.jpra.2024.06.010.

Ultrasonographic and Histological Correlation after Experimental Reconstruction of a Volumetric Muscle Loss Injury with Adipose Tissue.

Leiva-Cepas F, Benito-Ysamat A, Jimena I, Jimenez-Diaz F, Gil-Belmonte M, Ruz-Caracuel I Int J Mol Sci. 2021; 22(13).

PMID: 34206557 PMC: 8268690. DOI: 10.3390/ijms22136689.

References

Aguera E, Castilla S, Luque E, Jimena I, Ruz-Caracuel I, Leiva-Cepas F . Denervated muscle extract promotes recovery of muscle atrophy through activation of satellite cells. An experimental study. J Sport Health Sci. 2019; 8(1):23-31. PMC: 6349589. DOI: 10.1016/j.jshs.2017.05.007. View

Andersen D, Schroder H, Jensen C . Non-cultured adipose-derived CD45- side population cells are enriched for progenitors that give rise to myofibres in vivo. Exp Cell Res. 2008; 314(16):2951-64. DOI: 10.1016/j.yexcr.2008.06.018. View

Argentati C, Morena F, Bazzucchi M, Armentano I, Emiliani C, Martino S . Adipose Stem Cell Translational Applications: From Bench-to-Bedside. Int J Mol Sci. 2018; 19(11). PMC: 6275042. DOI: 10.3390/ijms19113475. View

Aurora A, Roe J, Corona B, Walters T . An acellular biologic scaffold does not regenerate appreciable de novo muscle tissue in rat models of volumetric muscle loss injury. Biomaterials. 2015; 67:393-407. DOI: 10.1016/j.biomaterials.2015.07.040. View

Bacou F, Andalousi R, Daussin P, Micallef J, Levin J, Chammas M . Transplantation of adipose tissue-derived stromal cells increases mass and functional capacity of damaged skeletal muscle. Cell Transplant. 2004; 13(2):103-11. View

Bierinx A, Sebille A . Mouse sectioned muscle regenerates following auto-grafting with muscle fragments: a new muscle precursor cells transfer?. Neurosci Lett. 2008; 431(3):211-4. DOI: 10.1016/j.neulet.2007.11.052. View

Choi J, Yoon H, Lee K, Choi Y, Yang S, Kim I . Exosomes from differentiating human skeletal muscle cells trigger myogenesis of stem cells and provide biochemical cues for skeletal muscle regeneration. J Control Release. 2015; 222:107-15. DOI: 10.1016/j.jconrel.2015.12.018. View

Cholok D, Lee E, Lisiecki J, Agarwal S, Loder S, Ranganathan K . Traumatic muscle fibrosis: From pathway to prevention. J Trauma Acute Care Surg. 2016; 82(1):174-184. PMC: 5177464. DOI: 10.1097/TA.0000000000001290. View

Cittadella Vigodarzere G, Mantero S . Skeletal muscle tissue engineering: strategies for volumetric constructs. Front Physiol. 2014; 5:362. PMC: 4170101. DOI: 10.3389/fphys.2014.00362. View

10.

Collinsworth A, Torgan C, Nagda S, Rajalingam R, Kraus W, Truskey G . Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch. Cell Tissue Res. 2000; 302(2):243-51. DOI: 10.1007/s004410000224. View

11.

Corona B, Greising S . Challenges to acellular biological scaffold mediated skeletal muscle tissue regeneration. Biomaterials. 2016; 104:238-46. DOI: 10.1016/j.biomaterials.2016.07.020. View

12.

Corona B, Garg K, Ward C, McDaniel J, Walters T, Rathbone C . Autologous minced muscle grafts: a tissue engineering therapy for the volumetric loss of skeletal muscle. Am J Physiol Cell Physiol. 2013; 305(7):C761-75. DOI: 10.1152/ajpcell.00189.2013. View

13.

Corona B, Wenke J, Ward C . Pathophysiology of Volumetric Muscle Loss Injury. Cells Tissues Organs. 2016; 202(3-4):180-188. DOI: 10.1159/000443925. View

14.

Corona B, Henderson B, Ward C, Greising S . Contribution of minced muscle graft progenitor cells to muscle fiber formation after volumetric muscle loss injury in wild-type and immune deficient mice. Physiol Rep. 2017; 5(7). PMC: 5392532. DOI: 10.14814/phy2.13249. View

15.

De Angelis L, Berghella L, Coletta M, Lattanzi L, Zanchi M, Ponzetto C . Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration. J Cell Biol. 1999; 147(4):869-78. PMC: 2156164. DOI: 10.1083/jcb.147.4.869. View

16.

De la Garza-Rodea A, van der Velde-van Dijke I, Boersma H, Goncalves M, van Bekkum D, de Vries A . Myogenic properties of human mesenchymal stem cells derived from three different sources. Cell Transplant. 2011; 21(1):153-73. DOI: 10.3727/096368911X580554. View

17.

Di Rocco G, Iachininoto M, Tritarelli A, Straino S, Zacheo A, Germani A . Myogenic potential of adipose-tissue-derived cells. J Cell Sci. 2006; 119(Pt 14):2945-52. DOI: 10.1242/jcs.03029. View

18.

Dunn A, Talovic M, Patel K, Patel A, Marcinczyk M, Garg K . Biomaterial and stem cell-based strategies for skeletal muscle regeneration. J Orthop Res. 2019; 37(6):1246-1262. DOI: 10.1002/jor.24212. View

19.

Ehrhardt J, Morgan J . Regenerative capacity of skeletal muscle. Curr Opin Neurol. 2005; 18(5):548-53. DOI: 10.1097/01.wco.0000177382.62156.82. View

20.

Eriksson A, Lindstrom M, Carlsson L, Thornell L . Hypertrophic muscle fibers with fissures in power-lifters; fiber splitting or defect regeneration?. Histochem Cell Biol. 2006; 126(4):409-17. DOI: 10.1007/s00418-006-0176-3. View