Missense Mutations in a Transmembrane Domain of the Komagataeibacter Xylinus BcsA Lead to Changes in Cellulose Synthesis

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2019 Sep 14

PMID 31514737

Citations 5

Authors

Luis Salgado

Silvia Blank

Reza Alipour Moghadam Esfahani

Janice L Strap

Dario Bonetta

Affiliations

Soon will be listed here.

Abstract

Background: Cellulose is synthesized by an array of bacterial species. Komagataeibacter xylinus is the best characterized as it produces copious amounts of the polymer extracellularly. Despite many advances in the past decade, the mechanisms underlying cellulose biosynthesis are not completely understood. Elucidation of these mechanisms is essential for efficient cellulose production in industrial applications.

Results: In an effort to gain a better understanding of cellulose biosynthesis and its regulation, cellulose crystallization was investigated in K. xylinus mutants resistant to an inhibitor of cellulose I formation, pellicin. Through the use of forward genetics and site-directed mutagenesis, A449T and A449V mutations in the K. xylinus BcsA protein were found to be important for conferring high levels of pellicin resistance. Phenotypic analysis of the bcsA and bcsA cultures revealed that the mutations affect cellulose synthesis rates and that cellulose crystallinity is affected in wet pellicles but not dry ones.

Conclusions: A449 is located in a predicted transmembrane domain of the BcsA protein suggesting that the structure of the transmembrane domain influences cellulose crystallization either by affecting the translocation of the nascent glucan chain or by allosterically altering protein-protein interactions.

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