Separation of Functional Subsets of Human T Cells by a Monoclonal Antibody

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Aug 1

PMID 315070

Citations 281

Authors

E L Reinherz

P C Kung

G Goldstein

S F Schlossman

Affiliations

Soon will be listed here.

Abstract

A monoclonal antibody was produced to human peripheral blood T cells. This hybridoma antibody, termed OKT4, was reactive by indirect immunofluorescence with only 55-60% of the peripheral blood T cell population (OKT4+) and unreactive with normal B cells, null cells, and macrophages. The OKT4- T cell population contained the previously described TH2+ subset that has been shown to contain cytotoxic/suppressor cells. With cell-sorter separation of OKT4+ and OKT4- cells, it was shown that these T cell subsets were functionally discrete. Both gave proliferative responses with concanavalin A, alloantigens, and phytohemagglutinin although OKT4+ cells were much more responsive to the latter. OKT4+ cells alone responded to soluble antigens whereas OKT4- cells alone were cytotoxic after alloantigenic sensitization of unfractionated T cells. However, both OKT4+ and OKT4- cells were required during sensitization for optimal development of cytotoxicity. These data suggest that the OKT4+ subset represents a helper population and that the OKT4- subset contains the cytotoxic effector population. OKT4 could be a valuable reagent for determining alterations of these functional subsets in human diseases.

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References

Reinherz E, Parkman R, Rappeport J, Rosen F, Schlossman S . Aberrations of suppressor T cells in human graft-versus-host disease. N Engl J Med. 1979; 300(19):1061-8. DOI: 10.1056/NEJM197905103001901. View

Reinherz E, Schlossman S . Con A-inducible suppression of MLC: evidence for mediation by the TH2 + T cell subset in man. J Immunol. 1979; 122(4):1335-41. View

Evans R, Lazarus H, Penta A, Schlossman S . Two functionally distinct subpopulations of human T cells that collaborate in the generation of cytotoxic cells responsible for cell-mediated lympholysis. J Immunol. 1978; 120(4):1423-8. View

Strelkauskas A, Schauf V, Wilson B, Chess L, Schlossman S . Isolation and characterization of naturally occurring subclasses of human peripheral blood T cells with regulatory functions. J Immunol. 1978; 120(4):1278-82. View

Reinherz E, Strelkauskas A, OBrien C, Schlossman S . Phenotypic and functional distinctions between the TH2+ and JRA+ T cell subsets in man. J Immunol. 1979; 123(1):83-6. View

Kierszenbaum F, Budzko D . Identification and characterization of a new subset of rat T lymphocytes. J Immunol. 1978; 121(1):166-71. View

Strelkauskas A, Callery R, McDowell J, Borel Y, Schlossman S . Direct evidence for loss of human suppressor cells during active autoimmune disease. Proc Natl Acad Sci U S A. 1978; 75(10):5150-4. PMC: 336282. DOI: 10.1073/pnas.75.10.5150. View

Moretta L, Ferrarini M, Mingari M, Moretta A, Webb S . Subpopulations of human T cells identified by receptors for immunoglobulins and mitogen responsiveness. J Immunol. 1976; 117(6):2171-4. View

Mendes N, Tolnai M, Silveira N, Gilbertsen R, METZGAR R . Technical aspects of the rosette tests used to detect human complement receptor (B) and sheep erythrocyte-binding (T) lymphocytes. J Immunol. 1973; 111(3):860-7. View

10.

Chess L, Macdermott R, Schlossman S . Immunologic functions of isolated human lymphocyte subpopulations. I. Quantitative isolation of human T and B cells and response to mitogens. J Immunol. 1974; 113(4):1113-21. View

11.

Sondel P, Chess L, Macdermott R, Schlossman S . Immunologic functions of isolated human lymphocyte subpopulations. III. Specific allogeneic lympholysis mediated by human T cells alone. J Immunol. 1975; 114(3):982-7. View

12.

Bonner W, Hulett H, Sweet R, Herzenberg L . Fluorescence activated cell sorting. Rev Sci Instrum. 1972; 43(3):404-9. DOI: 10.1063/1.1685647. View

13.

Margulies D, Kuehl W, Scharff M . Somatic cell hybridization of mouse myeloma cells. Cell. 1976; 8(3):405-15. DOI: 10.1016/0092-8674(76)90153-7. View

14.

CANTOR H, Boyse E . Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975; 141(6):1390-9. PMC: 2189854. DOI: 10.1084/jem.141.6.1390. View

15.

CANTOR H, Shen F, Boyse E . Separation of helper T cells from suppressor T cells expressing different Ly components. II. Activation by antigen: after immunization, antigen-specific suppressor and helper activities are mediated by distinct T-cell subclasses. J Exp Med. 1976; 143(6):1391-40. PMC: 2190222. DOI: 10.1084/jem.143.6.1391. View

16.

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View