Flow Cytometry: Retrospective, Fundamentals and Recent Instrumentation

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2012 Jan 25

PMID 22271369

Citations 71

Authors

Julien Picot

Coralie L Guerin

Caroline Le Van Kim

Chantal M Boulanger

Affiliations

Soon will be listed here.

Abstract

Flow cytometry is a complete technology given to biologists to study cellular populations with high precision. This technology elegantly combines sample dimension, data acquisition speed, precision and measurement multiplicity. Beyond the statistical aspect, flow cytometry offers the possibility to physically separate sub-populations. These performances come from the common endeavor of physicists, biophysicists, biologists and computer engineers, who succeeded, by providing new concepts, to bring flow cytometry to current maturity. The aim of this paper is to present a complete retrospective of the technique and remind flow cytometry fundamentals before focusing on recent commercial instrumentation.

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