Clonal Diversity of Haemophilus Influenzae Carriage Isolated from Under the Age of 6 years Children

Overview

Journal BMC Res Notes

Publisher Biomed Central

Specialties Biology
General Medicine

Date 2019 Sep 12

PMID 31506105

Citations 2

Authors

Fahimeh Shooraj

Bahman Mirzaei

Seyed Fazlollah Mousavi

Farzaneh Hosseini

Affiliations

Soon will be listed here.

Abstract

Objectives: Pharyngeal carriers such as H. influenzae seem to constitute the only reservoir and probably the only transmission vehicle of the invasive disease. The aims of this study were to estimate the prevalence of H. influenzae carriage, to characterize antibiotic susceptibility, and to explore genetic diversity of H. influenzae isolates. Sampling was carried out as nasopharynx swabs among children less than 6 years old volunteers. After traditional biochemical tests, isolates were confirmed by targeting omp6 sequence. Following the susceptibility tests, genomic diversity of strains was analyzed by Pulsed-Field Gel Electrophoresis procedure.

Results: Out of 328 nasopharynx swabs, 73 strains were identified as H. influenzae. Among H. influenzae isolates, resistance to chloramphenicol (42%) and ampicillin (43%) was observed. Levofloxacin is the most effective antibiotic and the least effect belonged to tetracycline. By genomic analysis of selected H. influenza, 28 PFGE patterns were achieved among which 11 patterns included at least 2 strains. All strains clustered into 25 different clones. The dendrogram analysis of the isolated H. influenzae strains showed that some of these strains had a clonal relationship and common genetic origin. According to our results, antibiotic resistance didn't show any significant correlation with the clonality of strains.

Citing Articles

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References

Saito M, Umeda A, Yoshida S . Subtyping of Haemophilus influenzae strains by pulsed-field gel electrophoresis. J Clin Microbiol. 1999; 37(7):2142-7. PMC: 85103. DOI: 10.1128/JCM.37.7.2142-2147.1999. View

Yano H, Suetake M, Kuga A, IRINODA K, Okamoto R, Kobayashi T . Pulsed-field gel electrophoresis analysis of nasopharyngeal flora in children attending a day care center. J Clin Microbiol. 2000; 38(2):625-9. PMC: 86161. DOI: 10.1128/JCM.38.2.625-629.2000. View

Cohen M . Epidemiology of drug resistance: implications for a post-antimicrobial era. Science. 1992; 257(5073):1050-5. DOI: 10.1126/science.257.5073.1050. View

Hasegawa K, Chiba N, Kobayashi R, Murayama S, Iwata S, Sunakawa K . Rapidly increasing prevalence of beta-lactamase-nonproducing, ampicillin-resistant Haemophilus influenzae type b in patients with meningitis. Antimicrob Agents Chemother. 2004; 48(5):1509-14. PMC: 400528. DOI: 10.1128/AAC.48.5.1509-1514.2004. View

Nazir J, Urban C, Mariano N, Burns J, Tommasulo B, Rosenberg C . Quinolone-resistant Haemophilus influenzae in a long-term care facility: clinical and molecular epidemiology. Clin Infect Dis. 2004; 38(11):1564-9. DOI: 10.1086/420820. View

Levy S, Marshall B . Antibacterial resistance worldwide: causes, challenges and responses. Nat Med. 2004; 10(12 Suppl):S122-9. DOI: 10.1038/nm1145. View

Brook I, Hausfeld J . Recovery of interfering bacteria in the nasopharynx following antimicrobial therapy of acute maxillary sinusitis with telithromycin or amoxicillin-clavulanate. Antimicrob Agents Chemother. 2005; 49(11):4793-4. PMC: 1280152. DOI: 10.1128/AAC.49.11.4793-4794.2005. View

Herrero A, Rodicio M, Gonzalez-Hevia M, Mendoza M . Molecular epidemiology of emergent multidrug-resistant Salmonella enterica serotype Typhimurium strains carrying the virulence resistance plasmid pUO-StVR2. J Antimicrob Chemother. 2005; 57(1):39-45. DOI: 10.1093/jac/dki400. View

Herschleb J, Ananiev G, Schwartz D . Pulsed-field gel electrophoresis. Nat Protoc. 2007; 2(3):677-84. DOI: 10.1038/nprot.2007.94. View

10.

Tristram S, Jacobs M, Appelbaum P . Antimicrobial resistance in Haemophilus influenzae. Clin Microbiol Rev. 2007; 20(2):368-89. PMC: 1865592. DOI: 10.1128/CMR.00040-06. View

11.

Mukundan D, Ecevit Z, Patel M, Marrs C, Gilsdorf J . Pharyngeal colonization dynamics of Haemophilus influenzae and Haemophilus haemolyticus in healthy adult carriers. J Clin Microbiol. 2007; 45(10):3207-17. PMC: 2045313. DOI: 10.1128/JCM.00492-07. View

12.

Rudan I, Boschi-Pinto C, Biloglav Z, Mulholland K, Campbell H . Epidemiology and etiology of childhood pneumonia. Bull World Health Organ. 2008; 86(5):408-16. PMC: 2647437. DOI: 10.2471/blt.07.048769. View

13.

Ladhani S, Heath P, Ramsay M, Slack M . Changes in antibiotic resistance rates of invasive Haemophilus influenzae isolates in England and Wales over the last 20 years. J Antimicrob Chemother. 2008; 62(4):776-9. DOI: 10.1093/jac/dkn274. View

14.

Azzari C, Moriondo M, Indolfi G, Massai C, Becciolini L, de Martino M . Molecular detection methods and serotyping performed directly on clinical samples improve diagnostic sensitivity and reveal increased incidence of invasive disease by Streptococcus pneumoniae in Italian children. J Med Microbiol. 2008; 57(Pt 10):1205-1212. PMC: 2884936. DOI: 10.1099/jmm.0.2008/000935-0. View

15.

Bae S, Lee J, Lee J, Kim E, Lee S, Yu J . Antimicrobial resistance in Haemophilus influenzae respiratory tract isolates in Korea: results of a nationwide acute respiratory infections surveillance. Antimicrob Agents Chemother. 2009; 54(1):65-71. PMC: 2798543. DOI: 10.1128/AAC.00966-09. View

16.

Andersson D, Hughes D . Antibiotic resistance and its cost: is it possible to reverse resistance?. Nat Rev Microbiol. 2010; 8(4):260-71. DOI: 10.1038/nrmicro2319. View

17.

Mojgani N, Rahbar M, Taqizadeh M, Ashtiani M, Mohammadzadeh M . Biotyping, capsular typing, and antibiotic resistance pattern of Haemophilus influenzae strains in Iran. Jpn J Infect Dis. 2011; 64(1):66-8. View

18.

Mohd-Zain Z, Kamsani N, Ismail I, Ahmad N . Antibiotic susceptibility profile of Haemophilus influenzae and transfer of co-trimoxazole resistance determinants. Trop Biomed. 2012; 29(3):372-80. View

19.

Moosazadeh Moghaddam M, Barjini K, Fasihi Ramandi M, Amani J . Investigation of the antibacterial activity of a short cationic peptide against multidrug-resistant Klebsiella pneumoniae and Salmonella typhimurium strains and its cytotoxicity on eukaryotic cells. World J Microbiol Biotechnol. 2013; 30(5):1533-40. DOI: 10.1007/s11274-013-1575-y. View

20.

Boroumand M, Irani S, Siadat S, Bouzari S . Molecular Detection of Genomic Islands Associated With Class 1 and 2 Integron in Haemophilus influenzae Isolated in Iran. Jundishapur J Microbiol. 2015; 8(4):e17249. PMC: 4449856. DOI: 10.5812/jjm.8(4)2015.17249. View