Unusual Evolutionary Conservation of 5S RRNA Pseudogenes in Aspergillus Nidulans: Similarity of the DNA Sequence Associated with the Pseudogenes with the Mouse Immunoglobulin Switch Region

Overview

Journal J Mol Evol

Specialty Biochemistry

Date 1988 Dec 1

PMID 3148732

Citations 4

Authors

P Borsuk

M Gniadkowski

E Bartnik

P P Stepien

Affiliations

Soon will be listed here.

Abstract

All Aspergillus nidulans 5S rRNA pseudogenes known so far are the result of integration of an approx. 0.2-kbp-long DNA sequence into the 5S rRNA genes. This sequence, called block C, is present in at least five copies in the A. nidulans genome and seems to be associated either with 5S rRNA genes or pseudogenes. In contrast to the 78% sequence conservation of the C-block in pseudogenes, the truncated 5' halves of the pseudogenes are very highly conserved (96.9-100%). We postulate that the 5S rRNA pseudogenes are still a subject of concerted evolution. The C-block sequence shows similarity to the switch region of the mouse heavy chain immunoglobulin gene. A characteristic motif GGGTGAG is repeated several times in both sequences; the sequence conservation is 63%.

Citing Articles

A Portrait of Ribosomal DNA Contacts with Hi-C Reveals 5S and 45S rDNA Anchoring Points in the Folded Human Genome.

Yu S, Lemos B Genome Biol Evol. 2016; 8(11):3545-3558.

PMID: 27797956 PMC: 5203791. DOI: 10.1093/gbe/evw257.

Multiple ITS haplotypes in the genome of the lichenized basidiomycete Cora inversa (Hygrophoraceae): fact or artifact?.

Lucking R, Lawrey J, Gillevet P, Sikaroodi M, Dal-Forno M, Berger S J Mol Evol. 2013; 78(2):148-62.

PMID: 24343640 DOI: 10.1007/s00239-013-9603-y.

Evolution of a large ribosomal RNA multigene family in filamentous fungi: birth and death of a concerted evolution paradigm.

Rooney A, Ward T Proc Natl Acad Sci U S A. 2005; 102(14):5084-9.

PMID: 15784739 PMC: 555991. DOI: 10.1073/pnas.0409689102.

Structure and evolution of 5S rRNA genes and pseudogenes in the genus Aspergillus.

Gniadkowski M, Fiett J, Borsuk P, Hoffman-Zacharska D, Stepien P, Bartnik E J Mol Evol. 1991; 33(2):175-8.

PMID: 1920453 DOI: 10.1007/BF02193632.

References

Bartoszewski S, Borsuk P, Kern I, Bartnik E . Microheterogeneity in Aspergillus nidulans 5S rRNA genes. Curr Genet. 1987; 11(6-7):571-3. DOI: 10.1007/BF00384621. View

Munro J, BURDON R, Leader D . Characterization of a human orphon 28 S ribosomal DNA. Gene. 1986; 48(1):65-70. DOI: 10.1016/0378-1119(86)90352-5. View

Sakonju S, Bogenhagen D, Brown D . A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region. Cell. 1980; 19(1):13-25. DOI: 10.1016/0092-8674(80)90384-0. View

Bartnik E, Kulik J, Nagiec M, Zagrodzka M . Cloning and mapping of fourteen different DNA fragments containing Aspergillus nidulans 5S rRNA genes. Acta Microbiol Pol. 1984; 33(1):5-10. View

Bartnik E, Bartoszewski S, Borsuk P, Empel J . Aspergillus nidulans 5S rRNA genes and pseudogenes. Curr Genet. 1986; 10(6):453-7. DOI: 10.1007/BF00419873. View

Vanin E . Processed pseudogenes: characteristics and evolution. Annu Rev Genet. 1985; 19:253-72. DOI: 10.1146/annurev.ge.19.120185.001345. View

Selker E, Yanofsky C, Driftmier K, Metzenberg R, RajBhandary U . Dispersed 5S RNA genes in N. crassa: structure, expression and evolution. Cell. 1981; 24(3):819-28. DOI: 10.1016/0092-8674(81)90107-0. View

Bogenhagen D, Sakonju S, Brown D . A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region. Cell. 1980; 19(1):27-35. DOI: 10.1016/0092-8674(80)90385-2. View

Tyler B . Transcription of Neurospora crassa 5 S rRNA genes requires a TATA box and three internal elements. J Mol Biol. 1987; 196(4):801-11. DOI: 10.1016/0022-2836(87)90406-2. View

10.

Kramer R, Philippsen P, Davis R . Divergent transcription in the yeast ribosomal RNA coding region as shown by hybridization to separated strands and sequence analysis of cloned DNA. J Mol Biol. 1978; 123(3):405-16. DOI: 10.1016/0022-2836(78)90087-6. View

11.

Childs G, Maxson R, Cohn R, Kedes L . Orphons: dispersed genetic elements derived from tandem repetitive genes of eucaryotes. Cell. 1981; 23(3):651-63. DOI: 10.1016/0092-8674(81)90428-1. View

12.

Jacq C, Miller J, Brownlee G . A pseudogene structure in 5S DNA of Xenopus laevis. Cell. 1977; 12(1):109-20. DOI: 10.1016/0092-8674(77)90189-1. View

13.

Nikaido T, Nakai S, Honjo T . Switch region of immunoglobulin Cmu gene is composed of simple tandem repetitive sequences. Nature. 1981; 292(5826):845-8. DOI: 10.1038/292845a0. View

14.

Bartnik E, Strugala K, Stepien P . Cloning and analysis of recombinant plasmids containing genes for Aspergillus nidulans 5 S rRNA. Curr Genet. 2013; 4(3):173-6. DOI: 10.1007/BF00420494. View

15.

Mao J, Appel B, Schaack J, Sharp S, Yamada H, Soll D . The 5S RNA genes of Schizosaccharomyces pombe. Nucleic Acids Res. 1982; 10(2):487-500. PMC: 326152. DOI: 10.1093/nar/10.2.487. View

16.

Long E, Dawid I . Repeated genes in eukaryotes. Annu Rev Biochem. 1980; 49:727-64. DOI: 10.1146/annurev.bi.49.070180.003455. View

17.

Chen M, Anne J, Volckaert G, Huysmans E, Vandenberghe A, De Wachter R . The nucleotide sequences of the 5 S rRNAs of seven molds and a yeast and their use in studying ascomycete phylogeny. Nucleic Acids Res. 1984; 12(12):4881-92. PMC: 318886. DOI: 10.1093/nar/12.12.4881. View

18.

Selker E, Free S, Metzenberg R, Yanofsky C . An isolated pseudogene related to the 5S RNA genes in Neurospora crassa. Nature. 1981; 294(5841):576-8. DOI: 10.1038/294576a0. View