Interference of Phosphane Copper (I) Complexes of β-carboline with Quorum Sensing Regulated Virulence Functions and Biofilm in Foodborne Pathogenic Bacteria: A First Report

Overview

Journal Saudi J Biol Sci

Specialty Biology

Date 2019 Sep 6

PMID 31485170

Citations 8

Authors

Nasser A Al-Shabib

Fohad Mabood Husain

Rais Ahmad Khan

Mohammad Shavez Khan

Mohammad Zubair Alam

Firoz Ahmad Ansari

Sameen Laeeq

Mohammad Zubair

Syed Ali Shahzad

Javed Masood Khan

Ali Alsalme

Iqbal Ahmad

Affiliations

Soon will be listed here.

Abstract

Foodborne pathogens are one of the major cause of food-related diseases and food poisoning. Bacterial biofilms and quorum sensing (QS) mechanism of cell-cell communication have also been found to be associated with several outbreaks of foodborne diseases and are great threat to food safety. Therefore, In the present study, we investigated the activity of three tetrahedrally coordinated copper(I) complexes against quorum sensing and biofilms of foodborne bacteria. All the three complexes demonstrated similar antimicrobial properties against the selected pathogens. Concentration below the MIC i.e. at sub-MICs all the three complexes interfered significantly with the quorum sensing regulated functions in (violacein), (elastase, pyocyanin and alginate production) and (prodigiosin). The complexes demonstrated potent broad-spectrum biofilm inhibition in , , , , and . Biofilm inhibition was visualized using SEM and CLSM images. Action of the copper(I) complexes on two key QS regulated functions contributing to biofilm formation i.e. EPS production and swarming motility was also studied and statistically significant reduction was recorded. These results could form the basis for development of safe anti-QS and anti-biofilm agents that can be utilized in the food industry as well as healthcare sector to prevent food-associated diseases.

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