YAP and TAZ Regulate Cell Volume

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2019 Sep 5

PMID 31481532

Citations 28

Authors

Nicolas A Perez-Gonzalez

Nash D Rochman

Kai Yao

Jiaxiang Tao

Minh-Tam Tran Le

Shannon Flanary

Lucia Sablich

Ben Toler

Eliana Crentsil

Felipe Takaesu

Bram Lambrus

Jessie Huang

Vivian Fu

Pragati Chengappa

Tia M Jones

Andrew J Holland

Steven An

Denis Wirtz

Ryan J Petrie

Kun-Liang Guan

Sean X Sun

Affiliations

Soon will be listed here.

Abstract

How mammalian cells regulate their physical size is currently poorly understood, in part due to the difficulty in accurately quantifying cell volume in a high-throughput manner. Here, using the fluorescence exclusion method, we demonstrate that the mechanosensitive transcriptional regulators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are regulators of single-cell volume. The role of YAP/TAZ in volume regulation must go beyond its influence on total cell cycle duration or cell shape to explain the observed changes in volume. Moreover, for our experimental conditions, volume regulation by YAP/TAZ is independent of mTOR. Instead, we find that YAP/TAZ directly impacts the cell division volume, and YAP is involved in regulating intracellular cytoplasmic pressure. Based on the idea that YAP/TAZ is a mechanosensor, we find that inhibiting myosin assembly and cell tension slows cell cycle progression from G1 to S. These results suggest that YAP/TAZ may be modulating cell volume in combination with cytoskeletal tension during cell cycle progression.

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