Phosphorylation of CEP83 by TTBK2 is Necessary for Cilia Initiation

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2019 Aug 29

PMID 31455668

Citations 34

Authors

Chien-Hui Lo

I-Hsuan Lin

T Tony Yang

Yen-Chun Huang

Barbara E Tanos

Po-Chun Chou

Chih-Wei Chang

Yeou-Guang Tsay

Jung-Chi Liao

Won-Jing Wang

Affiliations

Soon will be listed here.

Abstract

Primary cilia are microtubule-based organelles that play important roles in development and tissue homeostasis. Tau-tubulin kinase-2 (TTBK2) is genetically linked to spinocerebellar ataxia type 11, and its kinase activity is crucial for ciliogenesis. Although it has been shown that TTBK2 is recruited to the centriole by distal appendage protein CEP164, little is known about TTBK2 substrates associated with its role in ciliogenesis. Here, we perform superresolution microscopy and discover that serum starvation results in TTBK2 redistribution from the periphery toward the root of distal appendages. Our biochemical analyses uncover CEP83 as a bona fide TTBK2 substrate with four phosphorylation sites characterized. We also demonstrate that CEP164-dependent TTBK2 recruitment to distal appendages is required for subsequent CEP83 phosphorylation. Specifically, TTBK2-dependent CEP83 phosphorylation is important for early ciliogenesis steps, including ciliary vesicle docking and CP110 removal. In summary, our results reveal a molecular mechanism of kinase regulation in ciliogenesis and identify CEP83 as a key substrate of TTBK2 during cilia initiation.

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