Molecular Structure and Transformation of the Glucose Dehydrogenase Gene in Drosophila Melanogaster

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1988 Oct 1

PMID 3143620

Citations 5

Authors

R Whetten

E Organ

P Krasney

D Cox-Foster

D Cavener

Affiliations

Soon will be listed here.

Abstract

We have precisely mapped and sequenced the three 5' exons of the Drosophila melanogaster Gld gene and have identified the start sites for transcription and translation. The first exon is composed of 335 nucleotides and does not contain any putative translation start codons. The second exon is separated from the first exon by 8 kb and contains the Gld translation start codon. The inferred amino acid sequence of the amino terminus contains two unusual features: three tandem repeats of serine-alanine, and a relatively high density of cysteine residues. P element-mediated transformation experiments demonstrated that a 17.5-kb genomic fragment contains the functional and regulatory components of the Gld gene.

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