Structure and Transcription of the Drosophila Mulleri Alcohol Dehydrogenase Genes

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1985 Oct 11

PMID 2997730

Citations 38

Authors

J A Fischer

T Maniatis

Affiliations

Soon will be listed here.

Abstract

The D. melanogaster Adh gene is transcribed from two different promoters; a proximal (larval) promoter is active during late embryonic and larval stages, and a distal (adult) promoter is active primarily in third instar larvae and in adult flies (1). Genetic analyses suggest that several species of the mulleri subgroup (distant relatives of D. melanogaster) have two closely-linked Adh genes, Adh-1 and Adh-2, each of which expresses a different ADH protein (2). The temporal pattern of expression of Adh-1 and Adh-2 is similar to the expression of D. melanogaster Adh from the proximal and distal promoters (2,3,4). We are interested in the molecular basis for the pattern of Adh expression in the mulleri subgroup species and in the mechanism of the switch in Adh promoter utilization. For these reasons, we have studied the structure and transcription of the Adh locus of D. mulleri, a species of the mulleri subgroup. We show that the ADH-1 and ADH-2 proteins are expressed from two distinct genes separated by 2 kilobase pairs, and that Adh-1 and Adh-2 are transcribed in the expected temporal pattern. In addition, we find a pseudogene 1.2 kb upstream from Adh-2, which is transcribed in a temporal pattern similar to Adh-2.

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References

Neuberger M . Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells. EMBO J. 1983; 2(8):1373-8. PMC: 555285. DOI: 10.1002/j.1460-2075.1983.tb01594.x. View

Dudler R, Travers A . Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies. Cell. 1984; 38(2):391-8. DOI: 10.1016/0092-8674(84)90494-x. View

Schreier P, Cortese R . A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13. J Mol Biol. 1979; 129(1):169-72. DOI: 10.1016/0022-2836(79)90068-8. View

Lacy E, Maniatis T . The nucleotide sequence of a rabbit beta-globin pseudogene. Cell. 1980; 21(2):545-53. DOI: 10.1016/0092-8674(80)90492-4. View

Karn J, Brenner S, Barnett L, Cesareni G . Novel bacteriophage lambda cloning vector. Proc Natl Acad Sci U S A. 1980; 77(9):5172-6. PMC: 350019. DOI: 10.1073/pnas.77.9.5172. View

Efstratiadis A, Posakony J, Maniatis T, Lawn R, OConnell C, Spritz R . The structure and evolution of the human beta-globin gene family. Cell. 1980; 21(3):653-68. DOI: 10.1016/0092-8674(80)90429-8. View

Goldberg D . Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1980; 77(10):5794-8. PMC: 350157. DOI: 10.1073/pnas.77.10.5794. View

Frischauf A, Garoff H, Lehrach H . A subcloning strategy for DNA sequence analysis. Nucleic Acids Res. 1980; 8(23):5541-9. PMC: 324324. DOI: 10.1093/nar/8.23.5541. View

Steinmetz M, Moore K, Frelinger J, Sher B, Shen F, Boyse E . A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell. 1981; 25(3):683-92. DOI: 10.1016/0092-8674(81)90175-6. View

10.

Bingham P, Levis R, Rubin G . Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981; 25(3):693-704. DOI: 10.1016/0092-8674(81)90176-8. View

11.

LITTLE P . Globin pseudogenes. Cell. 1982; 28(4):683-4. DOI: 10.1016/0092-8674(82)90045-9. View

12.

Huang H, Crews S, Hood L . An immunoglobulin VH pseudogene. J Mol Appl Genet. 1981; 1(2):93-101. View

13.

Hong G . A systemic DNA sequencing strategy. J Mol Biol. 1982; 158(3):539-49. DOI: 10.1016/0022-2836(82)90213-3. View

14.

Karin M, Richards R . Human metallothionein genes--primary structure of the metallothionein-II gene and a related processed gene. Nature. 1982; 299(5886):797-802. DOI: 10.1038/299797a0. View

15.

Lemischka I, Sharp P . The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element. Nature. 1982; 300(5890):330-5. DOI: 10.1038/300330a0. View

16.

Snyder M, Hunkapiller M, Yuen D, SILVERT D, Fristrom J, Davidson N . Cuticle protein genes of Drosophila: structure, organization and evolution of four clustered genes. Cell. 1982; 29(3):1027-40. DOI: 10.1016/0092-8674(82)90466-4. View

17.

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

18.

Batterham P, Lovett J, Starmer W, Sullivan D . Differential regulation of duplicate alcohol dehydrogenase genes in Drosophila mojavensis. Dev Biol. 1983; 96(2):346-54. DOI: 10.1016/0012-1606(83)90172-0. View

19.

Everett R, Baty D, Chambon P . The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter. Nucleic Acids Res. 1983; 11(8):2447-64. PMC: 325895. DOI: 10.1093/nar/11.8.2447. View

20.

Lee M, Lewis S, Wilde C, Cowan N . Evolutionary history of a multigene family: an expressed human beta-tubulin gene and three processed pseudogenes. Cell. 1983; 33(2):477-87. DOI: 10.1016/0092-8674(83)90429-4. View