Lac Up-promoter Mutants with Increased Homology to the Consensus Promoter Sequence

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Dec 1

PMID 2999082

Citations 10

Authors

W Mandecki

R A Goldman

B S Powell

M H Caruthers

Affiliations

Soon will be listed here.

Abstract

Four lac promoter mutants were constructed. The mutations increased the homology between the lac promoter and the consensus promoter sequences by introducing the consensus -10 and -35 regions and the consensus spacing of 17 residues between these two regions. The promoter mutants were cloned into a pBR322-derivatized vector upstream from the lacZ gene, and levels of beta-galactosidase were an indication of promoter activity. All mutants exhibited higher activity than did the wild-type promoter.

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