Characterization of a Bifunctional Alginate Lyase As a New Member of the Polysaccharide Lyase Family 17 from a Marine Strain BP-2

Overview

Journal Biotechnol Lett

Specialties Biochemistry
Biotechnology

Date 2019 Aug 17

PMID 31418101

Citations 14

Authors

Guiyuan Huang

Shunhua Wen

Siming Liao

Qiaozhen Wang

Shihan Pan

Rongcan Zhang

Fu Lei

Wei Liao

Jie Feng

Shushi Huang

Affiliations

Soon will be listed here.

Abstract

Objectives: Bifunctional alginate lyase can efficiently saccharify alginate biomass and prepare functional oligosaccharides of alginate.

Results: A new BP-2 strain that produces alginate lyase was screened and identified from rotted Sargassum. A new alginate lyase, Alg17B, belonging to the polysaccharide lyase family 17, was isolated and purified from BP-2 fermentation broth by freeze-drying, dialysis, and ion exchange chromatography. The enzymatic properties of the purified lyase were investigated. The molecular weight of Alg17B was approximately 77 kDa, its optimum reaction temperature was 40-45 °C, and its optimum reaction pH was 7.5-8.0. The enzyme was relatively stable at pH 7.0-8.0, with a temperature range of 25-35 °C, and the specific activity of the purified enzyme reached 4036 U/mg. A low Na concentration stimulated Alg17B enzyme activity, but Ca, Zn, and other metal ions inhibited it. Substrate specificity analysis, thin-layer chromatography, and mass spectrometry showed that Alg17B is an alginate lyase that catalyses the hydrolysis of sodium alginate, polymannuronic acid (polyM) and polyguluronic acid to produce monosaccharides and low molecular weight oligosaccharides. Alg17B is also bifunctional, exhibiting both endolytic and exolytic activities toward alginate, and has a wide substrate utilization range with a preference for polyM.

Conclusions: Alg17B can be used to saccharify the main carbohydrate, alginate, in the ethanolic production of brown algae fuel as well as in preparing and researching oligosaccharides.

Citing Articles

Direct Preparation of Alginate Oligosaccharides from Brown Algae by an Algae-Decomposing Alginate Lyase AlyP18 from the Marine Bacterium A3.

Sun X, Zhang X, Zhang X, Wang X, Zhang X, Zhang Y Mar Drugs. 2024; 22(11).

PMID: 39590763 PMC: 11595925. DOI: 10.3390/md22110483.

Genome Analysis of a Potential Novel Species Secreting pH- and Thermo-Stable Alginate Lyase and Its Application in Producing Alginate Oligosaccharides.

Bao K, Yang M, Sun Q, Zhang K, Huang H Mar Drugs. 2024; 22(9).

PMID: 39330296 PMC: 11433491. DOI: 10.3390/md22090414.

A Recombinant Alginate Lyase Algt1 with Potential in Preparing Alginate Oligosaccharides at High-Concentration Substrate.

Liang Q, Huang Y, Liu Z, Xiao M, Ren X, Liu T Foods. 2023; 12(21).

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Characterization of a Novel Polysaccharide Lyase Family 5 Alginate Lyase with PolyM Substrate Specificity.

Zhou L, Meng Q, Zhang R, Jiang B, Liu X, Chen J Foods. 2022; 11(21).

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