Accelerated Mineralization on Nanofibers Via Non-thermal Atmospheric Plasma Assisted Glutamic Acid Templated Peptide Conjugation

Overview

Journal Regen Biomater

Specialty Biomedical Engineering

Date 2019 Aug 13

PMID 31404337

Citations 3

Authors

Gunnur Onak

Ozan Karaman

Affiliations

Soon will be listed here.

Abstract

Surface modification by non-thermal atmospheric plasma (NTAP) treatment can produce significantly higher carboxylic groups on the nanofibers (NF) surface, which potentially can increase biomineralization of NF via promoting glutamic acid (GLU) templated peptide conjugation. Herein, electrospun poly(lactide-co-glycolide) (PLGA) scaffolds were treated with NTAP and conjugated with GLU peptide followed by incubation in simulated body fluids for mineralization. The effect of NTAP treatment and GLU peptide conjugation on mineralization, surface wettability and roughness were investigated. The results showed that NTAP treatment significantly increased GLU peptide conjugation which consequently enhanced mineralization and mechanical properties of NTAP treated and peptide conjugated NF (GLU-pNF) compared to neat PLGA NF, NTAP treated NF (pNF) and GLU peptide conjugated NF (GLU-NF). The effect of surface modification on human bone marrow derived mesenchymal stem cells adhesion, proliferation and morphology was evaluated by cell proliferation assay and fluorescent microscopy. Results demonstrated that cellular adhesion and proliferation were significantly higher on GLU-pNF compared to NF, pNF and GLU-NF. In summary, NTAP treatment could be a promising modification technique to induce biomimetic peptide conjugation and biomineralization for bone tissue engineering applications.

Citing Articles

The Effect of Immobilization Methods of P9-4 Antimicrobial Peptide Onto Gelatin Methacrylate on Multidrug-Resistant Bacteria: A Comparative Study.

Pulat G, Celebi N, Bilgic E Macromol Biosci. 2024; 25(1):e2400324.

PMID: 39230389 PMC: 11727820. DOI: 10.1002/mabi.202400324.

Modified five times simulated body fluid for efficient biomimetic mineralization.

Fu K, Yang L, Gao N, Liu P, Xue B, He W Heliyon. 2024; 10(12):e32850.

PMID: 38975072 PMC: 11226902. DOI: 10.1016/j.heliyon.2024.e32850.

Supramolecular Peptide Nanofiber Hydrogels for Bone Tissue Engineering: From Multihierarchical Fabrications to Comprehensive Applications.

Hao Z, Li H, Wang Y, Hu Y, Chen T, Zhang S Adv Sci (Weinh). 2022; 9(11):e2103820.

PMID: 35128831 PMC: 9008438. DOI: 10.1002/advs.202103820.

Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds.

Permyakova E, Kiryukhantsev-Korneev P, Gudz K, Konopatsky A, Polcak J, Zhitnyak I Nanomaterials (Basel). 2019; 9(12).

PMID: 31842311 PMC: 6955782. DOI: 10.3390/nano9121769.

References

Sarvestani A, He X, Jabbari E . Osteonectin-derived peptide increases the modulus of a bone-mimetic nanocomposite. Eur Biophys J. 2007; 37(2):229-34. DOI: 10.1007/s00249-007-0198-3. View

Zhang Y, Venugopal J, El-Turki A, Ramakrishna S, Su B, Lim C . Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Biomaterials. 2008; 29(32):4314-22. DOI: 10.1016/j.biomaterials.2008.07.038. View

Barati D, Moeinzadeh S, Karaman O, Jabbari E . Time Dependence of Material Properties of Polyethylene Glycol Hydrogels Chain Extended with Short Hydroxy Acid Segments. Polymer (Guildf). 2014; 55(16):3894-3904. PMC: 4175514. DOI: 10.1016/j.polymer.2014.05.045. View

Dimitriou R, Mataliotakis G, Calori G, Giannoudis P . The role of barrier membranes for guided bone regeneration and restoration of large bone defects: current experimental and clinical evidence. BMC Med. 2012; 10:81. PMC: 3423057. DOI: 10.1186/1741-7015-10-81. View

Golda M, Brzychczy-Wloch M, Faryna M, Engvall K, Kotarba A . Oxygen plasma functionalization of parylene C coating for implants surface: nanotopography and active sites for drug anchoring. Mater Sci Eng C Mater Biol Appl. 2013; 33(7):4221-7. DOI: 10.1016/j.msec.2013.06.014. View

Tavafoghi M, Brodusch N, Gauvin R, Cerruti M . Hydroxyapatite formation on graphene oxide modified with amino acids: arginine versus glutamic acid. J R Soc Interface. 2016; 13(114):20150986. PMC: 4759803. DOI: 10.1098/rsif.2015.0986. View

Karaman O, Kelebek S, Demirci E, Ibis F, Ulu M, Ercan U . Synergistic Effect of Cold Plasma Treatment and RGD Peptide Coating on Cell Proliferation over Titanium Surfaces. Tissue Eng Regen Med. 2019; 15(1):13-24. PMC: 6171635. DOI: 10.1007/s13770-017-0087-5. View

Bas O, DAngella D, Baldwin J, Castro N, Wunner F, Saidy N . An Integrated Design, Material, and Fabrication Platform for Engineering Biomechanically and Biologically Functional Soft Tissues. ACS Appl Mater Interfaces. 2017; 9(35):29430-29437. DOI: 10.1021/acsami.7b08617. View

Deng X, Hao J, Wang C . Preparation and mechanical properties of nanocomposites of poly(D,L-lactide) with Ca-deficient hydroxyapatite nanocrystals. Biomaterials. 2001; 22(21):2867-73. DOI: 10.1016/s0142-9612(01)00031-x. View

10.

Zhang H, Mao X, Zhao D, Jiang W, Du Z, Li Q . Three dimensional printed polylactic acid-hydroxyapatite composite scaffolds for prefabricating vascularized tissue engineered bone: An in vivo bioreactor model. Sci Rep. 2017; 7(1):15255. PMC: 5681514. DOI: 10.1038/s41598-017-14923-7. View

11.

Chan B, Leong K . Scaffolding in tissue engineering: general approaches and tissue-specific considerations. Eur Spine J. 2008; 17 Suppl 4:467-79. PMC: 2587658. DOI: 10.1007/s00586-008-0745-3. View

12.

Birhanu G, Javar H, Seyedjafari E, Zandi-Karimi A, Dusti Telgerd M . An improved surface for enhanced stem cell proliferation and osteogenic differentiation using electrospun composite PLLA/P123 scaffold. Artif Cells Nanomed Biotechnol. 2017; 46(6):1274-1281. DOI: 10.1080/21691401.2017.1367928. View

13.

Mavis B, Demirtas T, Gumusderelioglu M, Gunduz G, Colak U . Synthesis, characterization and osteoblastic activity of polycaprolactone nanofibers coated with biomimetic calcium phosphate. Acta Biomater. 2009; 5(8):3098-111. DOI: 10.1016/j.actbio.2009.04.037. View

14.

Deng H, Wang S, Wang X, Du C, Shen X, Wang Y . Two competitive nucleation mechanisms of calcium carbonate biomineralization in response to surface functionality in low calcium ion concentration solution. Regen Biomater. 2016; 2(3):187-95. PMC: 4669016. DOI: 10.1093/rb/rbv010. View

15.

Lin Z, Solomon K, Zhang X, Pavlos N, Abel T, Willers C . In vitro evaluation of natural marine sponge collagen as a scaffold for bone tissue engineering. Int J Biol Sci. 2011; 7(7):968-77. PMC: 3157271. DOI: 10.7150/ijbs.7.968. View

16.

Cui W, Li X, Xie C, Zhuang H, Zhou S, Weng J . Hydroxyapatite nucleation and growth mechanism on electrospun fibers functionalized with different chemical groups and their combinations. Biomaterials. 2010; 31(17):4620-9. DOI: 10.1016/j.biomaterials.2010.02.050. View

17.

Hoyer B, Bernhardt A, Heinemann S, Stachel I, Meyer M, Gelinsky M . Biomimetically mineralized salmon collagen scaffolds for application in bone tissue engineering. Biomacromolecules. 2012; 13(4):1059-66. DOI: 10.1021/bm201776r. View

18.

Kim H, Che L, Ha Y, Ryu W . Mechanically-reinforced electrospun composite silk fibroin nanofibers containing hydroxyapatite nanoparticles. Mater Sci Eng C Mater Biol Appl. 2014; 40:324-35. DOI: 10.1016/j.msec.2014.04.012. View

19.

Karaman O, Kumar A, Moeinzadeh S, He X, Cui T, Jabbari E . Effect of surface modification of nanofibres with glutamic acid peptide on calcium phosphate nucleation and osteogenic differentiation of marrow stromal cells. J Tissue Eng Regen Med. 2013; 10(2):E132-46. DOI: 10.1002/term.1775. View

20.

Leszczak V, Place L, Franz N, Popat K, Kipper M . Nanostructured biomaterials from electrospun demineralized bone matrix: a survey of processing and crosslinking strategies. ACS Appl Mater Interfaces. 2014; 6(12):9328-37. DOI: 10.1021/am501700e. View