Synergistic Effect of Cold Plasma Treatment and RGD Peptide Coating on Cell Proliferation over Titanium Surfaces

Overview

Journal Tissue Eng Regen Med

Date 2019 Jan 4

PMID 30603531

Citations 10

Authors

Ozan Karaman

Seyfi Kelebek

Emine Afra Demirci

Fatma Ibis

Murat Ulu

Utku Kursat Ercan

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate the synergistic effect of cold atmospheric plasma (CAP) treatment and RGD peptide coating for enhancing cellular attachment and proliferation over titanium (Ti) surfaces. The surface structure of CAP-treated and RGD peptide-coated Ti discs were characterized by contact angle goniometer and atomic force microscopy. The effect of such surface modification on human bone marrow derived mesenchymal stem cells (hMSCs) adhesion and proliferation was assessed by cell proliferation and DNA content assays. Besides, hMSCs' adhesion and morphology on surface modified Ti discs were observed via fluorescent and scanning electron microscopy. RGD peptide coating following CAP treatment significantly enhanced cellular adhesion and proliferation among untreated, CAP-treated and RGD peptide-coated Ti discs. The treatment of Ti surfaces with CAP may contribute to improved RGD peptide coating, which enables increased cellular integrations with the Ti surfaces.

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References

Secchi A, Grigoriou V, Shapiro I, Cavalcanti-Adam E, Composto R, Ducheyne P . RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis. J Biomed Mater Res A. 2007; 83(3):577-84. DOI: 10.1002/jbm.a.31007. View

Klein M, Bijelic A, Ziebart T, Koch F, Kammerer P, Wieland M . Submicron scale-structured hydrophilic titanium surfaces promote early osteogenic gene response for cell adhesion and cell differentiation. Clin Implant Dent Relat Res. 2011; 15(2):166-75. DOI: 10.1111/j.1708-8208.2011.00339.x. View

Ferguson S, Broggini N, Wieland M, de Wild M, Rupp F, Geis-Gerstorfer J . Biomechanical evaluation of the interfacial strength of a chemically modified sandblasted and acid-etched titanium surface. J Biomed Mater Res A. 2006; 78(2):291-7. DOI: 10.1002/jbm.a.30678. View

Mustafa K, Wennerberg A, Wroblewski J, Hultenby K, Lopez B, Arvidson K . Determining optimal surface roughness of TiO(2) blasted titanium implant material for attachment, proliferation and differentiation of cells derived from human mandibular alveolar bone. Clin Oral Implants Res. 2001; 12(5):515-25. DOI: 10.1034/j.1600-0501.2001.120513.x. View

Germanier Y, Tosatti S, Broggini N, Textor M, Buser D . Enhanced bone apposition around biofunctionalized sandblasted and acid-etched titanium implant surfaces. A histomorphometric study in miniature pigs. Clin Oral Implants Res. 2006; 17(3):251-7. DOI: 10.1111/j.1600-0501.2005.01222.x. View

Bornstein M, Valderrama P, Jones A, Wilson T, Seibl R, Cochran D . Bone apposition around two different sandblasted and acid-etched titanium implant surfaces: a histomorphometric study in canine mandibles. Clin Oral Implants Res. 2008; 19(3):233-41. DOI: 10.1111/j.1600-0501.2007.01473.x. View

Yang G, He F, Yang X, Wang X, Zhao S . In vivo evaluation of bone-bonding ability of RGD-coated porous implant using layer-by-layer electrostatic self-assembly. J Biomed Mater Res A. 2008; 90(1):175-85. DOI: 10.1002/jbm.a.32055. View

Jemat A, Ghazali M, Razali M, Otsuka Y . Surface Modifications and Their Effects on Titanium Dental Implants. Biomed Res Int. 2015; 2015:791725. PMC: 4575991. DOI: 10.1155/2015/791725. View

Lang N, Salvi G, Huynh-Ba G, Ivanovski S, Donos N, Bosshardt D . Early osseointegration to hydrophilic and hydrophobic implant surfaces in humans. Clin Oral Implants Res. 2011; 22(4):349-56. DOI: 10.1111/j.1600-0501.2011.02172.x. View

10.

Vidal G, Blanchi T, Mieszawska A, Calabrese R, Rossi C, Vigneron P . Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides. Acta Biomater. 2012; 9(1):4935-43. PMC: 3508072. DOI: 10.1016/j.actbio.2012.09.003. View

11.

Karoussis I, Bragger U, Salvi G, Burgin W, Lang N . Effect of implant design on survival and success rates of titanium oral implants: a 10-year prospective cohort study of the ITI Dental Implant System. Clin Oral Implants Res. 2004; 15(1):8-17. DOI: 10.1111/j.1600-0501.2004.00983.x. View

12.

Smeets R, Stadlinger B, Schwarz F, Beck-Broichsitter B, Jung O, Precht C . Impact of Dental Implant Surface Modifications on Osseointegration. Biomed Res Int. 2016; 2016:6285620. PMC: 4958483. DOI: 10.1155/2016/6285620. View

13.

Puleo D, Nanci A . Understanding and controlling the bone-implant interface. Biomaterials. 1999; 20(23-24):2311-21. DOI: 10.1016/s0142-9612(99)00160-x. View

14.

Le Guehennec L, Lopez-Heredia M, Enkel B, Weiss P, Amouriq Y, Layrolle P . Osteoblastic cell behaviour on different titanium implant surfaces. Acta Biomater. 2008; 4(3):535-43. DOI: 10.1016/j.actbio.2007.12.002. View

15.

Mante F, Little K, Mante M, Rawle C, Baran G . Oxidation of titanium, RGD peptide attachment, and matrix mineralization rat bone marrow stromal cells. J Oral Implantol. 2005; 30(6):343-9. DOI: 10.1563/0.667.1. View

16.

Goodman S, Yao Z, Keeney M, Yang F . The future of biologic coatings for orthopaedic implants. Biomaterials. 2013; 34(13):3174-83. PMC: 3582840. DOI: 10.1016/j.biomaterials.2013.01.074. View

17.

Tugulu S, Lowe K, Scharnweber D, Schlottig F . Preparation of superhydrophilic microrough titanium implant surfaces by alkali treatment. J Mater Sci Mater Med. 2010; 21(10):2751-63. DOI: 10.1007/s10856-010-4138-x. View

18.

Schliephake H, Scharnweber D, Dard M, Rossler S, Sewing A, Meyer J . Effect of RGD peptide coating of titanium implants on periimplant bone formation in the alveolar crest. An experimental pilot study in dogs. Clin Oral Implants Res. 2002; 13(3):312-9. DOI: 10.1034/j.1600-0501.2002.130312.x. View

19.

Hirakawa Y, Jimbo R, Shibata Y, Watanabe I, Wennerberg A, Sawase T . Accelerated bone formation on photo-induced hydrophilic titanium implants: an experimental study in the dog mandible. Clin Oral Implants Res. 2012; 24 Suppl A100:139-44. DOI: 10.1111/j.1600-0501.2011.02401.x. View

20.

Ritts A, Li H, Yu Q, Xu C, Yao X, Hong L . Dentin surface treatment using a non-thermal argon plasma brush for interfacial bonding improvement in composite restoration. Eur J Oral Sci. 2010; 118(5):510-6. PMC: 2939732. DOI: 10.1111/j.1600-0722.2010.00761.x. View