Virus Lasers for Biological Detection

Overview

Journal Nat Commun

Specialty Biology

Date 2019 Aug 11

PMID 31399594

Citations 10

Authors

John E Hales

Guy Matmon

Paul A Dalby

John M Ward

Gabriel Aeppli

Affiliations

Soon will be listed here.

Abstract

The selective amplification of DNA in the polymerase chain reaction is used to exponentially increase the signal in molecular diagnostics for nucleic acids, but there are no analogous techniques for signal enhancement in clinical tests for proteins or cells. Instead, the signal from affinity-based measurements of these biomolecules depends linearly on the probe concentration. Substituting antibody-based probes tagged for fluorescent quantification with lasing detection probes would create a new platform for biomarker quantification based on optical rather than enzymatic amplification. Here, we construct a virus laser which bridges synthetic biology and laser physics, and demonstrate virus-lasing probes for biosensing. Our virus-lasing probes display an unprecedented > 10,000 times increase in signal from only a 50% increase in probe concentration, using fluorimeter-compatible optics, and can detect biomolecules at sub-100 fmol mL concentrations.

Citing Articles

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