Misaminoacylation and Transamidation Are Required for Protein Biosynthesis in Lactobacillus Bulgaricus

Overview

Journal Biochimie

Specialty Biochemistry

Date 1988 Mar 1

PMID 3139057

Citations 6

Authors

A Schon

H Hottinger

D Soll

Affiliations

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Abstract

Aminoacylation studies with Lactobacillus bulgaricus show that this organism possesses glutamyl-tRNA synthetase activity; however, glutamyl-tRNA synthetase activity cannot be demonstrated. Instead, Glu-tRNAGln, which is formed by glutamyl-tRNA synthetase, is amidated by a specific amidotransferase to Gln-tRNAGln. The amide donor in this reaction is glutamine. Thus, Gln-tRNAGln in this organism is not formed by direct glutaminylation of tRNAGln, but instead by a pathway which involves misaminoacylation and transamidation.

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