Evaluation of Polymorphisms in Inflammatory Mediator and Cellular Adhesion Genes As Risk Factors for Feline Infectious Peritonitis

Overview

Journal J Feline Med Surg

Specialty Veterinary Medicine

Date 2019 Aug 3

PMID 31373532

Citations 5

Authors

Helen Kedward-Dixon

Emi N Barker

Severine Tasker

Anja Kipar

Christopher R Helps

Affiliations

Soon will be listed here.

Abstract

Objectives: Feline infectious peritonitis (FIP) is a high mortality infectious disease. Single nucleotide polymorphisms (SNPs) in the genes encoding interferon gamma (), tumour necrosis factor alpha () and dendritic cell-specific intercellular adhesion molecule-grabbing non-integrin (DC-SIGN; ) have been associated with increased and decreased risk of developing FIP. This study was designed to determine whether these associations were present in a UK population of pedigree cats using samples from cats euthanased with a confirmed diagnosis (FIP, n = 22; non-FIP, n = 10) or clinically healthy cats over 11 years of age (n = 3).

Methods: DNA was extracted from tissue (n = 32) or blood (n = 3) and PCR performed for regions of and . PCR amplicons were sequenced, each SNP genotype was determined, and genotype/allele frequency for each SNP and FIP status were compared.

Results: No significant association was found between the genotype and FIP status for any SNP analysed. There was a trend for the heterozygous CT genotype at both g.401 and IFNG g.408 to be associated with FIP ( = 0.13), but this genotype was also found in a substantial proportion of non-FIP cats. There was also a trend for the heterozygous CT genotype at g.428 to be associated with FIP ( = 0.06), although most cats with FIP had the CC genotype at this locus. No associations were found between any allele at g.-421, g.1900, g.2276, g.2392 and g.2713 and FIP.

Conclusions And Relevance: The use of the and SNPs described to predict the risk of FIP cannot currently be recommended.

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