Whole Blood Cytokine Profiles in Cats Infected by Feline Coronavirus and Healthy Non-FCoV Infected Specific Pathogen-free Cats

Overview

Journal J Feline Med Surg

Specialty Veterinary Medicine

Date 2006 Jun 17

PMID 16777454

Citations 31

Authors

Maria E Gelain

Marina Meli

Saverio Paltrinieri

Affiliations

Soon will be listed here.

Abstract

In this study, the cytokine profiles of clinically healthy cats naturally infected with feline coronavirus (FCoV), of cats with feline infectious peritonitis (FIP) and of specific pathogen-free (SPF) cats were investigated in whole blood using a traditional reverse-transcriptase polymerase chain reaction (RT-PCR) assay and a semi-quantitative method of analysis based on computerised quantification of positive bands. The low inter-assay coefficient of variation recorded demonstrated that this method is highly repeatable. Compared with SPF cats, cytokine production was upregulated in most of the samples from FCoV-positive non-symptomatic cats. The appearance of a case of FIP in the cattery was associated with an increased expression of cytokines, in particular there was an increased production of IL-1beta and IFN-gamma, suggesting that these cytokines might protect infected cats from the disease. This hypothesis was also supported by the low levels of IFN-gamma recorded in blood from cats with FIP.

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