Suppression of the Long Non-coding RNA MALAT-1 Impairs the Growth and Migration of Human Tongue Squamous Cell Carcinoma SCC4 Cells

Overview

Journal Arch Med Sci

Specialty General Medicine

Date 2019 Jul 31

PMID 31360193

Citations 8

Authors

Xu Han

Zixiao Xu

Gang Tian

Zhen Tang

Jianyong Gao

Yibo Wei

Xiaogang Xu

Affiliations

Soon will be listed here.

Abstract

Introduction: Aberrant expression of long non-coding RNAs (lncRNAs) is associated with metastasis and poor prognosis in patients with various cancer types. However, few studies have assessed lncRNAs in oral squamous cell carcinoma (OSCC). This study aimed to investigate the expression and impact of lncRNAs in OSCC.

Material And Methods: Real-time PCR analysis was used to examine the expression of four lncRNAs, MALAT-1, UCA1, BC200 and SRA, in 14 OSCC and adjacent normal tissue pairs. The impact of MALAT-1 suppression by siRNA on the proliferation, apoptosis, anchorage-independent growth and migration of the human tongue carcinoma cell line SSC4 was also determined.

Results: MALAT-1 levels were significantly higher in the OSCC tissue than in the normal tissues ( < 0.004); no significant differences in UCA1, BC200 or SRA RNA levels were observed. Knockdown of MALAT-1 by siRNA significantly suppressed proliferation of SSC4 cells ( < 0.004) and enhanced their apoptosis ( < 0.001). In addition, siRNA-mediated suppression of MALAT-1 inhibited SSC4 cell colony formation ( < 0.001) and migration ( < 0.004).

Conclusions: Elevated expression of MALAT-1 in OSCC may play a role in tumorigenesis and/or metastasis. Further studies are necessary to identify the mechanism by which MALAT-1 influences SCC4 growth and migration and validate its increased expression in OSCC patients.

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