Silver Nanoparticles Alter Epithelial Basement Membrane Integrity, Cell Adhesion Molecule Expression, and TGF-β1 Secretion

Overview

Journal Nanomedicine

Publisher Elsevier

Specialties Biomedical Engineering
Biotechnology

Date 2019 Jul 28

PMID 31351238

Citations 7

Authors

Megan E Martin

Denise K Reaves

Breanna Jeffcoat

Jeffrey R Enders

Lindsey M Costantini

Susan T Yeyeodu

Diane Botta

Terrance J Kavanagh

Jodie M Fleming

Affiliations

Soon will be listed here.

Abstract

Silver nanoparticles (AgNPs) are widely used in consumer and pharmaceutical products due to their antipathogenic properties. However, safety concerns have been raised due to their bioactive properties. While reports have demonstrated AgNPs can embed within the extracellular matrix, their effects on basement membrane (BM) production, integrin engagement, and tissue-integrity are not well-defined. This study analyzed the effects of AgNPs on BM production, composition and integrin/focal adhesion interactions in representative lung, esophageal, breast and colorectal epithelia models. A multidisciplinary approach including focused proteomics, QPCR arrays, pathway analyses, and immune-based, structural and functional assays was used to identify molecular and physiological changes in cell adhesions and the BM induced by acute and chronic AgNP exposure. Dysregulated targets included CD44 and transforming growth factor-beta, two proteins frequently altered during pathogenesis. Results indicate AgNP exposure interferes with BM and cell adhesion dynamics, and provide insight into the mechanisms of AgNP-induced disruption of epithelial physiology.

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References

Akter M, Sikder M, Rahman M, Ullah A, Hossain K, Banik S . A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives. J Adv Res. 2018; 9:1-16. PMC: 6057238. DOI: 10.1016/j.jare.2017.10.008. View

Xu Y, Tang H, Liu J, Wang H, Liu Y . Evaluation of the adjuvant effect of silver nanoparticles both in vitro and in vivo. Toxicol Lett. 2013; 219(1):42-8. DOI: 10.1016/j.toxlet.2013.02.010. View

Jiao Q, Bi L, Ren Y, Song S, Wang Q, Wang Y . Advances in studies of tyrosine kinase inhibitors and their acquired resistance. Mol Cancer. 2018; 17(1):36. PMC: 5817861. DOI: 10.1186/s12943-018-0801-5. View

Mailleux A, Overholtzer M, Brugge J . Lumen formation during mammary epithelial morphogenesis: insights from in vitro and in vivo models. Cell Cycle. 2008; 7(1):57-62. DOI: 10.4161/cc.7.1.5150. View

Schaeublin N, Braydich-Stolle L, Schrand A, Miller J, Hutchison J, Schlager J . Surface charge of gold nanoparticles mediates mechanism of toxicity. Nanoscale. 2011; 3(2):410-20. DOI: 10.1039/c0nr00478b. View

Chanmee T, Ontong P, Mochizuki N, Kongtawelert P, Konno K, Itano N . Excessive hyaluronan production promotes acquisition of cancer stem cell signatures through the coordinated regulation of Twist and the transforming growth factor β (TGF-β)-Snail signaling axis. J Biol Chem. 2014; 289(38):26038-26056. PMC: 4176217. DOI: 10.1074/jbc.M114.564120. View

Derynck R, Weinberg R . EMT and Cancer: More Than Meets the Eye. Dev Cell. 2019; 49(3):313-316. PMC: 7672963. DOI: 10.1016/j.devcel.2019.04.026. View

Mytych J, Zebrowski J, Lewinska A, Wnuk M . Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol. 2016; 54(2):1285-1300. PMC: 5310673. DOI: 10.1007/s12035-016-9688-6. View

Cao Z, Livas T, Kyprianou N . Anoikis and EMT: Lethal "Liaisons" during Cancer Progression. Crit Rev Oncog. 2016; 21(3-4):155-168. PMC: 5451151. DOI: 10.1615/CritRevOncog.2016016955. View

10.

Rath G, Hussain T, Chauhan G, Garg T, Goyal A . Collagen nanofiber containing silver nanoparticles for improved wound-healing applications. J Drug Target. 2015; 24(6):520-9. DOI: 10.3109/1061186X.2015.1095922. View

11.

Pure E, Assoian R . Rheostatic signaling by CD44 and hyaluronan. Cell Signal. 2009; 21(5):651-5. PMC: 2650006. DOI: 10.1016/j.cellsig.2009.01.024. View

12.

Rinna A, Magdolenova Z, Hudecova A, Kruszewski M, Refsnes M, Dusinska M . Effect of silver nanoparticles on mitogen-activated protein kinases activation: role of reactive oxygen species and implication in DNA damage. Mutagenesis. 2014; 30(1):59-66. DOI: 10.1093/mutage/geu057. View

13.

Cukierman E, Pankov R, Stevens D, Yamada K . Taking cell-matrix adhesions to the third dimension. Science. 2001; 294(5547):1708-12. DOI: 10.1126/science.1064829. View

14.

Frankson R, Yu Z, Bai Y, Li Q, Zhang R, Zhang Z . Therapeutic Targeting of Oncogenic Tyrosine Phosphatases. Cancer Res. 2017; 77(21):5701-5705. PMC: 5827927. DOI: 10.1158/0008-5472.CAN-17-1510. View

15.

Langhans S . Three-Dimensional Cell Culture Models in Drug Discovery and Drug Repositioning. Front Pharmacol. 2018; 9:6. PMC: 5787088. DOI: 10.3389/fphar.2018.00006. View

16.

Groger C, Grubinger M, Waldhor T, Vierlinger K, Mikulits W . Meta-analysis of gene expression signatures defining the epithelial to mesenchymal transition during cancer progression. PLoS One. 2012; 7(12):e51136. PMC: 3519484. DOI: 10.1371/journal.pone.0051136. View

17.

Huaux F . Emerging Role of Immunosuppression in Diseases Induced by Micro- and Nano-Particles: Time to Revisit the Exclusive Inflammatory Scenario. Front Immunol. 2018; 9:2364. PMC: 6252316. DOI: 10.3389/fimmu.2018.02364. View

18.

Kononenko V, Narat M, Drobne D . Nanoparticle interaction with the immune system. Arh Hig Rada Toksikol. 2015; 66(2):97-108. DOI: 10.1515/aiht-2015-66-2582. View

19.

Vila L, Marcos R, Hernandez A . Long-term effects of silver nanoparticles in caco-2 cells. Nanotoxicology. 2017; 11(6):771-780. DOI: 10.1080/17435390.2017.1355997. View

20.

Fabregat I, Malfettone A, Soukupova J . New Insights into the Crossroads between EMT and Stemness in the Context of Cancer. J Clin Med. 2016; 5(3). PMC: 4810108. DOI: 10.3390/jcm5030037. View