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ADNP Prompts the Cisplatin-resistance of Bladder Cancer Via TGF-β-mediated Epithelial-mesenchymal Transition (EMT) Pathway

Overview
Journal J Cancer
Specialty Oncology
Date 2021 Aug 2
PMID 34335928
Citations 10
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Abstract

Activity-dependent neuroprotective protein (ADNP) is vital for embryonic development and brain formation. Besides, the upregulated expression of ADNP enhances tumorigenesis in some human tumors like bladder cancer (BC). However, the potential roles of ADNP in drug resistance and the related mechanisms in BC is unknown. We performed this study to elucidate the influence of ADNP in the chemoresistance of BC and tried to explore the underlying molecular mechanism. The expressions of ADNP in BC from progression and non-progression patient specimens were measured by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). experiments including colony formation, cell counting kit-8 (CCK-8), wound healing, and tumorigenesis assay were performed to explore the effects of ADNP on chemoresistance of BC. The impacts of ADNP on TGF-β/Smad signaling pathways were explored by western blot. Our results showed that the expression of ADNP mRNA and protein were significantly upregulated in BC tissues of the patients who suffered tumor-progression via RT-PCR and western blot. Cox regression survival analysis revealed that patients with high ADNP expression closely linked to shorter tumor-free survival. ADNP downregulation in BC showed more sensitive to cisplatin , while ADNP overexpression showed the opposite results. Additionally, we confirmed that ADNP promoted cell migration and EMT, thereby inducing cisplatin resistance, which may be related to TGF-β / Smad signaling pathway.

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References
1.
Wang B, Chen Q, Cao Y, Ma X, Yin C, Jia Y . LGR5 Is a Gastric Cancer Stem Cell Marker Associated with Stemness and the EMT Signature Genes NANOG, NANOGP8, PRRX1, TWIST1, and BMI1. PLoS One. 2016; 11(12):e0168904. PMC: 5199039. DOI: 10.1371/journal.pone.0168904. View

2.
Zamostiano R, Pinhasov A, Gelber E, Steingart R, Seroussi E, Giladi E . Cloning and characterization of the human activity-dependent neuroprotective protein. J Biol Chem. 2000; 276(1):708-14. DOI: 10.1074/jbc.M007416200. View

3.
Deng G, Chen L, Zhang Y, Fan S, Li W, Lu J . Fucosyltransferase 2 induced epithelial-mesenchymal transition via TGF-β/Smad signaling pathway in lung adenocarcinaoma. Exp Cell Res. 2018; 370(2):613-622. DOI: 10.1016/j.yexcr.2018.07.026. View

4.
Wilson C, Lukowicz R, Merchant S, Valquier-Flynn H, Caballero J, Sandoval J . Quantitative and Qualitative Assessment Methods for Biofilm Growth: A Mini-review. Res Rev J Eng Technol. 2018; 6(4). PMC: 6133255. View

5.
Sakai S, Ohhata T, Kitagawa K, Uchida C, Aoshima T, Niida H . Long Noncoding RNA Acts as a Smad3 Cofactor to Facilitate TGFβ/Smad Signaling and Promote Epithelial-Mesenchymal Transition. Cancer Res. 2019; 79(11):2821-2838. DOI: 10.1158/0008-5472.CAN-18-3210. View