Protein Amounts of the MYC Transcription Factor Determine Germinal Center B Cell Division Capacity

Overview

Journal Immunity

Publisher Cell Press

Specialty Allergy & Immunology

Date 2019 Jul 28

PMID 31350178

Citations 82

Authors

Shlomo Finkin

Harald Hartweger

Thiago Y Oliveira

Ervin E Kara

Michel C Nussenzweig

Affiliations

Soon will be listed here.

Abstract

High-affinity B cell selection in the germinal center (GC) is governed by signals delivered by follicular helper T (Tfh) cells to B cells. Selected B cells undergo clonal expansion and affinity maturation in the GC dark zone in direct proportion to the amount of antigen they capture and present to Tfh cells in the light zone. Here, we examined the mechanisms whereby Tfh cells program the number of GC B cell divisions. Gene expression analysis revealed that Tfh cells induce Myc expression in light-zone B cells in direct proportion to antigen capture. Conditional Myc haplo-insufficiency or overexpression combined with cell division tracking showed that MYC expression produces a metabolic reservoir in selected light-zone B cells that is proportional to the number of cell divisions in the dark zone. Thus, MYC constitutes the GC B cell division timer that when deregulated leads to emergence of B cell lymphoma.

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