Stanniocalcin-1 Mediates Negative Regulatory Action of Epidermal Layer on Expression of Matrix-related Genes in Dermal Fibroblasts

Overview

Journal Biofactors

Publisher IOS Press

Specialty Biochemistry

Date 2019 Jul 27

PMID 31348577

Citations 4

Authors

Tomonobu Ezure

Satoshi Amano

Affiliations

Soon will be listed here.

Abstract

Dermal-epidermal interaction plays a role in many pathophysiological processes, such as tumor invasion and psoriasis, as well as wound healing, and is mediated at least in part by secretory factors. In this study, we investigated the factor(s) involved. We found that stanniocalcin-1 (STC1), a cytokine, is expressed at the basal layer of epidermis. Knockdown of STC1 with siRNA in HaCaT cells decreased matrix metalloproteinase 1 (MMP1) expression, suggesting that STC1 serves as an autocrine factor, maintaining MMP1 mRNA expression in the epidermal layer. In dermal fibroblasts, STC1 increased MMP1 mRNA expression and decreased collagen1A1 and elastin mRNA expression. These actions were inhibited by SP600125, a jun kinase (JNK) inhibitor. Nuclear translocation of AP-1, a downstream signal of JNK, was implicated in the actions of STC1. In a coculture system of HaCaT cells and fibroblasts, used as a model of dermal-epidermal interaction, knockdown of STC1 in HaCaT cells with siRNA reduced the negative effects (i.e., induction of MMP1 and decrease of collagen1A1 and elastin) of STC1 on fibroblasts. These results suggest that STC1 secreted from the epidermal layer is a mediator of dermal-epidermal interaction.

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References

Cornmark L, Lonne G, Jogi A, Larsson C . Protein kinase Cα suppresses the expression of STC1 in MDA-MB-231 breast cancer cells. Tumour Biol. 2011; 32(5):1023-30. DOI: 10.1007/s13277-011-0205-2. View

Ma X, Gu L, Li H, Gao Y, Li X, Shen D . Hypoxia-induced overexpression of stanniocalcin-1 is associated with the metastasis of early stage clear cell renal cell carcinoma. J Transl Med. 2015; 13:56. PMC: 4337255. DOI: 10.1186/s12967-015-0421-4. View

Sarapio E, Souza S, Vogt E, Rocha D, Fabres R, Trapp M . Effects of stanniocalcin hormones on rat brown adipose tissue metabolism under fed and fasted conditions. Mol Cell Endocrinol. 2019; 485:81-87. DOI: 10.1016/j.mce.2019.02.004. View

Shirakawa M, Fujiwara Y, Sugita Y, Moon J, Takiguchi S, Nakajima K . Assessment of stanniocalcin-1 as a prognostic marker in human esophageal squamous cell carcinoma. Oncol Rep. 2011; 27(4):940-6. PMC: 3583603. DOI: 10.3892/or.2011.1607. View

Qin Z, Voorhees J, Fisher G, Quan T . Age-associated reduction of cellular spreading/mechanical force up-regulates matrix metalloproteinase-1 expression and collagen fibril fragmentation via c-Jun/AP-1 in human dermal fibroblasts. Aging Cell. 2014; 13(6):1028-37. PMC: 4326925. DOI: 10.1111/acel.12265. View

Zlot C, Ingle G, Hongo J, Yang S, Sheng Z, Schwall R . Stanniocalcin 1 is an autocrine modulator of endothelial angiogenic responses to hepatocyte growth factor. J Biol Chem. 2003; 278(48):47654-9. DOI: 10.1074/jbc.M301353200. View

Ghaffari A, Kilani R, Ghahary A . Keratinocyte-conditioned media regulate collagen expression in dermal fibroblasts. J Invest Dermatol. 2008; 129(2):340-7. DOI: 10.1038/jid.2008.253. View

Chang A, Doherty J, Huschtscha L, Redvers R, Restall C, Reddel R . STC1 expression is associated with tumor growth and metastasis in breast cancer. Clin Exp Metastasis. 2014; 32(1):15-27. DOI: 10.1007/s10585-014-9687-9. View

Ezure T, Amano S . Negative regulation of dermal fibroblasts by enlarged adipocytes through release of free fatty acids. J Invest Dermatol. 2011; 131(10):2004-9. DOI: 10.1038/jid.2011.145. View

10.

Ezure T, Amano S . Stanniocalcin-1 mediates negative regulatory action of epidermal layer on expression of matrix-related genes in dermal fibroblasts. Biofactors. 2019; 45(6):944-949. PMC: 6916204. DOI: 10.1002/biof.1547. View

11.

Ghahary A, Ghaffari A . Role of keratinocyte-fibroblast cross-talk in development of hypertrophic scar. Wound Repair Regen. 2008; 15 Suppl 1:S46-53. DOI: 10.1111/j.1524-475X.2007.00225.x. View

12.

Yoshiko Y, Aubin J . Stanniocalcin 1 as a pleiotropic factor in mammals. Peptides. 2004; 25(10):1663-9. DOI: 10.1016/j.peptides.2004.04.015. View

13.

Bernat-Peguera A, Simon-Extremera P, da Silva-Diz V, Lopez de Munain M, Diaz-Gil L, Penin R . PDGFR-induced autocrine SDF-1 signaling in cancer cells promotes metastasis in advanced skin carcinoma. Oncogene. 2019; 38(25):5021-5037. PMC: 6756210. DOI: 10.1038/s41388-019-0773-y. View

14.

Rohani M, Parks W . Matrix remodeling by MMPs during wound repair. Matrix Biol. 2015; 44-46:113-21. DOI: 10.1016/j.matbio.2015.03.002. View

15.

Du Y, Gu X, Li L, Gao F . The diagnostic value of circulating stanniocalcin-1 mRNA in non-small cell lung cancer. J Surg Oncol. 2011; 104(7):836-40. DOI: 10.1002/jso.21948. View

16.

Park B, Hwang E, Seo S, Cho J, Yang J, Yi T . Eucalyptus globulus extract protects against UVB-induced photoaging by enhancing collagen synthesis via regulation of TGF-β/Smad signals and attenuation of AP-1. Arch Biochem Biophys. 2017; 637:31-39. DOI: 10.1016/j.abb.2017.11.007. View

17.

Kanellis J, Bick R, Garcia G, Truong L, Tsao C, Etemadmoghadam D . Stanniocalcin-1, an inhibitor of macrophage chemotaxis and chemokinesis. Am J Physiol Renal Physiol. 2003; 286(2):F356-62. DOI: 10.1152/ajprenal.00138.2003. View

18.

Ahmed S, Gogal Jr R, Walsh J . A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. J Immunol Methods. 1994; 170(2):211-24. DOI: 10.1016/0022-1759(94)90396-4. View

19.

Reichert O, Kolbe L, Terstegen L, Staeb F, Wenck H, Schmelz M . UV radiation induces CXCL5 expression in human skin. Exp Dermatol. 2015; 24(4):309-12. DOI: 10.1111/exd.12652. View

20.

McCudden C, Majewski A, Chakrabarti S, Wagner G . Co-localization of stanniocalcin-1 ligand and receptor in human breast carcinomas. Mol Cell Endocrinol. 2004; 213(2):167-72. DOI: 10.1016/j.mce.2003.10.042. View