[Correlation Between Gut Microbiota and Behavior Symptoms in Children with Autism Spectrum Disorder]

Overview

Journal Zhongguo Dang Dai Er Ke Za Zhi

Specialty Pediatrics

Date 2019 Jul 19

PMID 31315765

Citations 12

Authors

Rui-Hao Zhao

Peng-Yuan Zheng

Si-Meng Liu

You-Cai Tang

En-Yao Li

Zhen-Yu Sun

Miao-Miao Jiang

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the composition of gut microbiota and its correlation with the severity of behavior symptoms in children with autism spectrum disorder (ASD).

Methods: A total of 30 children with ASD were enrolled as the ASD group, and 20 healthy children matched for age and sex were enrolled as the healthy control group. Related clinical data were analyzed. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene in fecal samples were sequenced. The severity of behavior symptoms in children with ASD was assessed using the autism behavior checklist. The Spearman's correlation analysis was used to investigate the correlation between gut microbiota and the severity of behavior symptoms in children with ASD.

Results: There was a significant difference in the composition of gut microbiota between the two groups. Compared with the healthy control group, the ASD group had significant reductions in Shannon index and Shannoneven index (P<0.05), as well as a significant reduction in the percentage of Firmicutes and a significant increase in the percentage of Acidobacteria in feces (P<0.05). In the ASD group, the dominant bacteria were Megamonas, Megasphaera, and Barnesiella, while in the healthy control group, the dominant bacteria were Eubacterium_rectale_group, Ezakiella, and Streptococcus. In the children with ASD, the abundance of Megamonas was positively correlated with the scores of health/physical/behavior and language communication (P<0.05).

Conclusions: The development of ASD and the severity of behavior symptoms are closely associated with the composition of gut microbiota.

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References

Huang H, Liu J, Yu Y, Mo L, Ge R, Zhang H . Regulation of TWIK-related potassium channel-1 (Trek1) restitutes intestinal epithelial barrier function. Cell Mol Immunol. 2015; 13(1):110-8. PMC: 4711681. DOI: 10.1038/cmi.2014.137. View

Slattery J, MacFabe D, Frye R . The Significance of the Enteric Microbiome on the Development of Childhood Disease: A Review of Prebiotic and Probiotic Therapies in Disorders of Childhood. Clin Med Insights Pediatr. 2016; 10:91-107. PMC: 5063840. DOI: 10.4137/CMPed.S38338. View

Fombonne E . Epidemiology of pervasive developmental disorders. Pediatr Res. 2009; 65(6):591-8. DOI: 10.1203/PDR.0b013e31819e7203. View

Wang H, Wang P, Wang X, Wan Y, Liu Y . Butyrate enhances intestinal epithelial barrier function via up-regulation of tight junction protein Claudin-1 transcription. Dig Dis Sci. 2012; 57(12):3126-35. DOI: 10.1007/s10620-012-2259-4. View

Lai M, Lombardo M, Baron-Cohen S . Autism. Lancet. 2013; 383(9920):896-910. DOI: 10.1016/S0140-6736(13)61539-1. View

Fitzgerald M . The clinical utility of the ADI-R and ADOS in diagnosing autism. Br J Psychiatry. 2017; 211(2):117. DOI: 10.1192/bjp.211.2.117. View

Kelly J, Minuto C, Cryan J, Clarke G, Dinan T . Cross Talk: The Microbiota and Neurodevelopmental Disorders. Front Neurosci. 2017; 11:490. PMC: 5605633. DOI: 10.3389/fnins.2017.00490. View

Kieler I, Kamal S, Vitger A, Nielsen D, Lauridsen C, Bjornvad C . Gut microbiota composition may relate to weight loss rate in obese pet dogs. Vet Med Sci. 2017; 3(4):252-262. PMC: 5677773. DOI: 10.1002/vms3.80. View

Maynard C, Elson C, Hatton R, Weaver C . Reciprocal interactions of the intestinal microbiota and immune system. Nature. 2012; 489(7415):231-41. PMC: 4492337. DOI: 10.1038/nature11551. View

10.

Sherry C, Kim S, Dilger R, Bauer L, Moon M, Tapping R . Sickness behavior induced by endotoxin can be mitigated by the dietary soluble fiber, pectin, through up-regulation of IL-4 and Th2 polarization. Brain Behav Immun. 2010; 24(4):631-40. PMC: 2856791. DOI: 10.1016/j.bbi.2010.01.015. View

11.

Baio J, Wiggins L, Christensen D, Maenner M, Daniels J, Warren Z . Prevalence of Autism Spectrum Disorder Among Children Aged 8 Years - Autism and Developmental Disabilities Monitoring Network, 11 Sites, United States, 2014. MMWR Surveill Summ. 2018; 67(6):1-23. PMC: 5919599. DOI: 10.15585/mmwr.ss6706a1. View

12.

Hadjkacem I, Ayadi H, Turki M, Yaich S, Khemekhem K, Walha A . Prenatal, perinatal and postnatal factors associated with autism spectrum disorder. J Pediatr (Rio J). 2016; 92(6):595-601. DOI: 10.1016/j.jped.2016.01.012. View

13.

de La Cochetiere M, Piloquet H, des Robert C, Darmaun D, Galmiche J, Roze J . Early intestinal bacterial colonization and necrotizing enterocolitis in premature infants: the putative role of Clostridium. Pediatr Res. 2004; 56(3):366-70. DOI: 10.1203/01.PDR.0000134251.45878.D5. View

14.

Sekirov I, Russell S, Antunes L, Finlay B . Gut microbiota in health and disease. Physiol Rev. 2010; 90(3):859-904. DOI: 10.1152/physrev.00045.2009. View

15.

Geng H, Shu S, Dong J, Li H, Xu C, Han Y . Association study of gut flora in Wilson's disease through high-throughput sequencing. Medicine (Baltimore). 2018; 97(31):e11743. PMC: 6081054. DOI: 10.1097/MD.0000000000011743. View

16.

. Structure, function and diversity of the healthy human microbiome. Nature. 2012; 486(7402):207-14. PMC: 3564958. DOI: 10.1038/nature11234. View

17.

Kamp-Becker I, Albertowski K, Becker J, Ghahreman M, Langmann A, Mingebach T . Diagnostic accuracy of the ADOS and ADOS-2 in clinical practice. Eur Child Adolesc Psychiatry. 2018; 27(9):1193-1207. DOI: 10.1007/s00787-018-1143-y. View

18.

Rigsbee L, Agans R, Shankar V, Kenche H, Khamis H, Michail S . Quantitative profiling of gut microbiota of children with diarrhea-predominant irritable bowel syndrome. Am J Gastroenterol. 2012; 107(11):1740-51. DOI: 10.1038/ajg.2012.287. View

19.

Hesla H, Stenius F, Jaderlund L, Nelson R, Engstrand L, Alm J . Impact of lifestyle on the gut microbiota of healthy infants and their mothers—the ALADDIN birth cohort. FEMS Microbiol Ecol. 2014; 90(3):791-801. DOI: 10.1111/1574-6941.12434. View

20.

Rose S, Bennuri S, Davis J, Wynne R, Slattery J, Tippett M . Butyrate enhances mitochondrial function during oxidative stress in cell lines from boys with autism. Transl Psychiatry. 2018; 8(1):42. PMC: 5804031. DOI: 10.1038/s41398-017-0089-z. View