Cytotoxic Effects of Auraptene Against a Human Malignant Glioblastoma Cell Line

Overview

Journal Avicenna J Phytomed

Publisher Mashhad University of Medical Sciences

Date 2019 Jul 17

PMID 31309072

Citations 16

Authors

Amir R Afshari

Mostafa Karimi Roshan

Mohammad Soukhtanloo

Ahmad Ghorbani

Farzad Rahmani

Mohammad Jalili-Nik

Mohammad Mahdi Vahedi

Azar Hoseini

Hamid R Sadeghnia

Hamid Mollazadeh

Seyed Hadi Mousavi

Affiliations

Soon will be listed here.

Abstract

Objective: Glioblastoma multiforme (GBM) is the deadliest type of primary brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of auraptene on U87 GBM cell line.

Materials And Methods: The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrations of auraptene (0-400μg/ml). Apoptosis was evaluated by sub-G1 peak in cell cycle analysis of propidium-iodide- stained nuclei. Moreover, to determine the Ba, , , , , and genes expression, we used real-time polymerase chain reaction (RT-PCR).

Results: The results revealed that auraptene reduced the viability of U87 cells concentration- and time-dependently with IC values of 108.9 and 79.17μg/ml obtained for 24 and 48-hr treatments, respectively. Also, sub-G1 population was significantly increased following 24 (p<0.05 and p<0.001) and 48 (p<0.001) hours of treatment. The quantitative real-time RT-PCR showed an up-regulation in , , , and but a down-regulation in and genes expression.

Conclusion: This study showed that auraptene triggered apoptosis probably through Bax/Bcl-2 regulation, blocked cell cycle progression and inhibited proliferation in U87 GBM cells. Taken together, auraptene can be utilized as an effective natural medicine against GBM, after complementary studies.

Citing Articles

Auraptene inhibits migration, invasion and metastatic behavior of human malignant glioblastoma cells: An and study.

Mousavi S, Jalili-Nik M, Soukhtanloo M, Soltani A, Abbasinezhad-Moud F, Mollazadeh H Avicenna J Phytomed. 2024; 14(3):349-364.

PMID: 39086858 PMC: 11287035. DOI: 10.22038/AJP.2023.23586.

Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-87 MG cell line.

Abadi B, Abdesheikhi J, Sedghy F, Mahmoodi M, Fallah H Avicenna J Phytomed. 2024; 14(2):166-176.

PMID: 38966629 PMC: 11221771. DOI: 10.22038/AJP.2023.22935.

Niosome-encapsulated auraptene reduced the mRNA expression of and genes in human retina-derived RPE cell line.

Vahidi A, Khosravi T, Dastaviz F, Sheikh Arabi M, Khosravi A, Oladnabi M Int J Ophthalmol. 2024; 17(6):1028-1035.

PMID: 38895680 PMC: 11144767. DOI: 10.18240/ijo.2024.06.06.

New Avenues and Major Achievements in Phytocompounds Research for Glioblastoma Therapy.

Majchrzak-Celinska A, Studzinska-Sroka E Molecules. 2024; 29(7).

PMID: 38611962 PMC: 11013944. DOI: 10.3390/molecules29071682.

gum exerts cytotoxic effects against human malignant glioblastoma multiforme .

Afshari A, Mousavi S, Mousavi G, Moghadam S, Maghrouni A, Javid H Res Pharm Sci. 2022; 17(5):585-593.

PMID: 36386486 PMC: 9661685. DOI: 10.4103/1735-5362.355215.

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