Long Non-coding RNA HOTAIR/microRNA-206 Sponge Regulates STC2 and Further Influences Cell Biological Functions in Head and Neck Squamous Cell Carcinoma

Overview

Journal Cell Prolif

Date 2019 Jul 13

PMID 31297902

Citations 36

Authors

Tiancheng Li

Yao Qin

Zhen Zhen

Hong Shen

Tiechuan Cong

Erik Schiferle

Shuifang Xiao

Affiliations

Soon will be listed here.

Abstract

Objective: It is essential to characterize underlying molecular mechanism associated with head and neck squamous cell carcinoma (HNSCC) and identify promising therapeutic targets. Herein, we explored role of homeobox transcript antisense RNA (HOTAIR) in HNSCC to regulate stanniocalcin-2 (STC2) by sponging microRNA-206 (miR-206).

Methods: HNSCC-related differentially expressed genes and regulation network amongst HOTAIR, miR-206 and STC2 were identified. Next, effect of HOTAIR on cell biological functions of HNSCC was identified after transfection of cells with HOTAIR overexpressed plasmids or siRNA against HOTAIR. PI3K/AKT signalling pathway-related gene expression was measured after miR-206 and STC2 were suppressed. Cell invasion, migration and proliferation were assessed. Finally, tumour growth was assessed to determine the effects of HOTAIR/miR-206/STC2 axis in vivo.

Results: HOTAIR specifically bound to miR-206 and miR-206 targeted STC2. Downregulated HOTAIR or upregulated miR-206 suppressed HNSCC cell proliferation, invasion and migration. miR-206 inhibited PI3K/AKT signalling pathway by down-regulating STC2. Besides, silenced HOTAIR or overexpressed miR-206 repressed the tumour growth of nude mice with HNSCC.

Conclusion: HOTAIR regulated HNSCC cell biological functions by binding to miR-206 through STC2.

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