Effect of Inactivated As Non-pathogenic Particles on the Severity of Pneumonia Caused by Respiratory Syncytial Virus Infection in Mice

Overview

Journal Toxicol Rep

Date 2019 Jun 28

PMID 31245279

Citations 1

Authors

Aki Miyauchi

Wataru Watanabe

Toshi Akashi

Seiko Hashiguchi

Hiroki Yoshida

Chihiro Sugita

Masahiko Kurokawa

Affiliations

Soon will be listed here.

Abstract

The severity of pneumonia in respiratory syncytial virus (RSV) infection is strongly related to host immune response and external factors such as bacteria and environmental chemicals. We investigated the effect of inactivated (ISP) as non-pathogenic particles on the severity of pneumonia in RSV-infected mice. Mice were intranasally exposed to ISP before RSV infection. On day 5 post-infection, we examined tissues, virus titer, and infiltrated cells in the lungs. The ISP did not cause significant histopathological effects in the lungs of RSV infected mice, but reduced virus titer. It also reduced the ratio of lymphocyte infiltration into the lungs and consequently the ratio of macrophage increased. In addition, we found that ISP increased RANTES level in bronchoalveolar lavage fluid from RSV-infected mice on day 1 post-infection, but reduced type I interferon levels. Thus, ISP did not exacerbate pneumonia in RSV infection, rather, it might mildly reduce the severity. We characterize and discuss the inherent activity of ISP as non-pathogenic particles inducing the role of RANTES on the pneumonia in RSV infection.

Citing Articles

Cost-Effectiveness of Bivalent Respiratory Syncytial Virus Prefusion F (RSVpreF) Vaccine During Pregnancy for Prevention of Respiratory Syncytial Virus Among Infants in Argentina.

Rey-Ares L, Averin A, Zuccarino N, Vega C, Kutrieb E, Quinn E Infect Dis Ther. 2024; 13(11):2363-2376.

PMID: 39365506 PMC: 11499499. DOI: 10.1007/s40121-024-01055-5.

References

Nguyen D, Louwen R, Elberse K, van Amerongen G, Yuksel S, Luijendijk A . Streptococcus pneumoniae Enhances Human Respiratory Syncytial Virus Infection In Vitro and In Vivo. PLoS One. 2015; 10(5):e0127098. PMC: 4430531. DOI: 10.1371/journal.pone.0127098. View

Maghazachi A, Schall T . C-C chemokines induce the chemotaxis of NK and IL-2-activated NK cells. Role for G proteins. J Immunol. 1994; 153(11):4969-77. View

Hament J, Aerts P, Fleer A, van Dijk H, Harmsen T, Kimpen J . Direct binding of respiratory syncytial virus to pneumococci: a phenomenon that enhances both pneumococcal adherence to human epithelial cells and pneumococcal invasiveness in a murine model. Pediatr Res. 2005; 58(6):1198-203. DOI: 10.1203/01.pdr.0000188699.55279.1b. View

Bogaert D, de Groot R, Hermans P . Streptococcus pneumoniae colonisation: the key to pneumococcal disease. Lancet Infect Dis. 2004; 4(3):144-54. DOI: 10.1016/S1473-3099(04)00938-7. View

Farrag M, Almajhdi F . Human Respiratory Syncytial Virus: Role of Innate Immunity in Clearance and Disease Progression. Viral Immunol. 2015; 29(1):11-26. DOI: 10.1089/vim.2015.0098. View

Visan L, Rouleau N, Proust E, Peyrot L, Donadieu A, Ochs M . Antibodies to PcpA and PhtD protect mice against Streptococcus pneumoniae by a macrophage- and complement-dependent mechanism. Hum Vaccin Immunother. 2017; 14(2):489-494. PMC: 5806646. DOI: 10.1080/21645515.2017.1403698. View

Vissers M, Ahout I, van den Kieboom C, van der Gaast-de Jongh C, Groh L, Cremers A . High pneumococcal density correlates with more mucosal inflammation and reduced respiratory syncytial virus disease severity in infants. BMC Infect Dis. 2016; 16:129. PMC: 4794819. DOI: 10.1186/s12879-016-1454-x. View

Villalta F, Zhang Y, Bibb K, Kappes J, Lima M . The cysteine-cysteine family of chemokines RANTES, MIP-1alpha, and MIP-1beta induce trypanocidal activity in human macrophages via nitric oxide. Infect Immun. 1998; 66(10):4690-5. PMC: 108576. DOI: 10.1128/IAI.66.10.4690-4695.1998. View

Hussell T, Openshaw P . Intracellular IFN-gamma expression in natural killer cells precedes lung CD8+ T cell recruitment during respiratory syncytial virus infection. J Gen Virol. 1998; 79 ( Pt 11):2593-601. DOI: 10.1099/0022-1317-79-11-2593. View

10.

Koppe U, Hogner K, Doehn J, Muller H, Witzenrath M, Gutbier B . Streptococcus pneumoniae stimulates a STING- and IFN regulatory factor 3-dependent type I IFN production in macrophages, which regulates RANTES production in macrophages, cocultured alveolar epithelial cells, and mouse lungs. J Immunol. 2011; 188(2):811-7. DOI: 10.4049/jimmunol.1004143. View

11.

Watanabe W, Shimizu T, Hino A, Kurokawa M . Effects of decabrominated diphenyl ether (DBDE) on developmental immunotoxicity in offspring mice. Environ Toxicol Pharmacol. 2011; 26(3):315-9. DOI: 10.1016/j.etap.2008.06.004. View

12.

Watanabe W, Shimizu T, Sawamura R, Hino A, Konno K, Hirose A . Effects of tetrabromobisphenol A, a brominated flame retardant, on the immune response to respiratory syncytial virus infection in mice. Int Immunopharmacol. 2010; 10(4):393-7. DOI: 10.1016/j.intimp.2009.12.014. View

13.

Avadhanula V, Wang Y, Portner A, Adderson E . Nontypeable Haemophilus influenzae and Streptococcus pneumoniae bind respiratory syncytial virus glycoprotein. J Med Microbiol. 2007; 56(Pt 9):1133-1137. DOI: 10.1099/jmm.0.47086-0. View

14.

Lee C, Peng H, Yang P, Liou J, Liao C, Day Y . C-C Chemokine Ligand-5 is critical for facilitating macrophage infiltration in the early phase of liver ischemia/reperfusion injury. Sci Rep. 2017; 7(1):3698. PMC: 5473895. DOI: 10.1038/s41598-017-03956-7. View

15.

Holberg C, Wright A, Martinez F, RAY C, Taussig L, LEBOWITZ M . Risk factors for respiratory syncytial virus-associated lower respiratory illnesses in the first year of life. Am J Epidemiol. 1991; 133(11):1135-51. DOI: 10.1093/oxfordjournals.aje.a115826. View

16.

Takeshita T, Watanabe W, Toyama S, Hayashi Y, Honda S, Sakamoto S . Effect of brazilian propolis on exacerbation of respiratory syncytial virus infection in mice exposed to tetrabromobisphenol a, a brominated flame retardant. Evid Based Complement Alternat Med. 2013; 2013:698206. PMC: 3819786. DOI: 10.1155/2013/698206. View

17.

Opitz B, van Laak V, Eitel J, Suttorp N . Innate immune recognition in infectious and noninfectious diseases of the lung. Am J Respir Crit Care Med. 2010; 181(12):1294-309. DOI: 10.1164/rccm.200909-1427SO. View

18.

MacDonald N, Hall C, Suffin S, Alexson C, Harris P, Manning J . Respiratory syncytial viral infection in infants with congenital heart disease. N Engl J Med. 1982; 307(7):397-400. DOI: 10.1056/NEJM198208123070702. View

19.

Duttweiler L, Nadal D, Frey B . Pulmonary and systemic bacterial co-infections in severe RSV bronchiolitis. Arch Dis Child. 2004; 89(12):1155-7. PMC: 1719766. DOI: 10.1136/adc.2004.049551. View

20.

Hashiguchi S, Yoshida H, Akashi T, Komemoto K, Ueda T, Ikarashi Y . Titanium dioxide nanoparticles exacerbate pneumonia in respiratory syncytial virus (RSV)-infected mice. Environ Toxicol Pharmacol. 2015; 39(2):879-86. DOI: 10.1016/j.etap.2015.02.017. View