Revealing Brain Pathologies with Multimodal Visible Light Optical Coherence Microscopy and Fluorescence Imaging

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2019 Jun 27

PMID 31240898

Citations 9

Authors

Antonia Lichtenegger

Johanna Gesperger

Barbara Kiesel

Martina Muck

Pablo Eugui

Danielle J Harper

Matthias Salas

Marco Augustin

Conrad W Merkle

Christoph K Hitzenberger

Georg Widhalm

Adelheid Woehrer

Bernhard Baumann

Affiliations

Soon will be listed here.

Abstract

We present a multimodal visible light optical coherence microscopy (OCM) and fluorescence imaging (FI) setup. Specification and phantom measurements were performed to characterize the system. Two applications in neuroimaging were investigated. First, curcumin-stained brain slices of a mouse model of Alzheimer's disease were examined. Amyloid-beta plaques were identified based on the fluorescence of curcumin, and coregistered morphological images of the brain tissue were provided by the OCM channel. Second, human brain tumor biopsies retrieved intraoperatively were imaged prior to conventional neuropathologic work-up. OCM revealed the three-dimensional structure of the brain parenchyma, and FI added the tumor tissue-specific contrast. Attenuation coefficients computed from the OCM data and the florescence intensity values were analyzed and showed a statistically significant difference for 5-aminolevulinic acid (5-ALA)-positive and -negative brain tissues. OCM findings correlated well with malignant hot spots within brain tumor biopsies upon histopathology. The combination of OCM and FI seems to be a promising optical imaging modality providing complementary contrast for applications in the field of neuroimaging.

Citing Articles

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References

Colditz M, van Leyen K, Jeffree R . Aminolevulinic acid (ALA)-protoporphyrin IX fluorescence guided tumour resection. Part 2: theoretical, biochemical and practical aspects. J Clin Neurosci. 2012; 19(12):1611-6. DOI: 10.1016/j.jocn.2012.03.013. View

Assayag O, Grieve K, Devaux B, Harms F, Pallud J, Chretien F . Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography. Neuroimage Clin. 2013; 2:549-57. PMC: 3778248. DOI: 10.1016/j.nicl.2013.04.005. View

Gora M, Suter M, Tearney G, Li X . Endoscopic optical coherence tomography: technologies and clinical applications [Invited]. Biomed Opt Express. 2017; 8(5):2405-2444. PMC: 5480489. DOI: 10.1364/BOE.8.002405. View

Bohringer H, Lankenau E, Stellmacher F, Reusche E, Huttmann G, Giese A . Imaging of human brain tumor tissue by near-infrared laser coherence tomography. Acta Neurochir (Wien). 2009; 151(5):507-17. PMC: 3085760. DOI: 10.1007/s00701-009-0248-y. View

Harper D, Augustin M, Lichtenegger A, Eugui P, Reyes C, Glosmann M . White light polarization sensitive optical coherence tomography for sub-micron axial resolution and spectroscopic contrast in the murine retina. Biomed Opt Express. 2018; 9(5):2115-2129. PMC: 5946775. DOI: 10.1364/BOE.9.002115. View

Roetzer T, Leskovar K, Peter N, Furtner J, Muck M, Augustin M . Evaluating cellularity and structural connectivity on whole brain slides using a custom-made digital pathology pipeline. J Neurosci Methods. 2018; 311:215-221. PMC: 6269083. DOI: 10.1016/j.jneumeth.2018.10.029. View

Stummer W, Tonn J, Goetz C, Ullrich W, Stepp H, Bink A . 5-Aminolevulinic acid-derived tumor fluorescence: the diagnostic accuracy of visible fluorescence qualities as corroborated by spectrometry and histology and postoperative imaging. Neurosurgery. 2013; 74(3):310-9. PMC: 4206350. DOI: 10.1227/NEU.0000000000000267. View

Cole G, Morihara T, Lim G, Yang F, Begum A, Frautschy S . NSAID and antioxidant prevention of Alzheimer's disease: lessons from in vitro and animal models. Ann N Y Acad Sci. 2005; 1035:68-84. DOI: 10.1196/annals.1332.005. View

Jankowsky J, Fadale D, Anderson J, Xu G, Gonzales V, Jenkins N . Mutant presenilins specifically elevate the levels of the 42 residue beta-amyloid peptide in vivo: evidence for augmentation of a 42-specific gamma secretase. Hum Mol Genet. 2003; 13(2):159-70. DOI: 10.1093/hmg/ddh019. View

10.

Drexler W, Liu M, Kumar A, Kamali T, Unterhuber A, Leitgeb R . Optical coherence tomography today: speed, contrast, and multimodality. J Biomed Opt. 2014; 19(7):071412. DOI: 10.1117/1.JBO.19.7.071412. View

11.

Vermeer K, Mo J, Weda J, Lemij H, de Boer J . Depth-resolved model-based reconstruction of attenuation coefficients in optical coherence tomography. Biomed Opt Express. 2014; 5(1):322-37. PMC: 3891343. DOI: 10.1364/BOE.5.000322. View

12.

Gambichler T, Moussa G, Sand M, Sand D, Altmeyer P, Hoffmann K . Applications of optical coherence tomography in dermatology. J Dermatol Sci. 2005; 40(2):85-94. DOI: 10.1016/j.jdermsci.2005.07.006. View

13.

Ju M, Huang C, Wahl D, Jian Y, Sarunic M . Visible light sensorless adaptive optics for retinal structure and fluorescence imaging. Opt Lett. 2018; 43(20):5162-5165. DOI: 10.1364/OL.43.005162. View

14.

Feroldi F, Verlaan M, Knaus H, Davidoiu V, Vugts D, van Dongen G . High resolution combined molecular and structural optical imaging of colorectal cancer in a xenograft mouse model. Biomed Opt Express. 2019; 9(12):6186-6204. PMC: 6491025. DOI: 10.1364/BOE.9.006186. View

15.

Finke M, Kantelhardt S, Schlaefer A, Bruder R, Lankenau E, Giese A . Automatic scanning of large tissue areas in neurosurgery using optical coherence tomography. Int J Med Robot. 2012; 8(3):327-36. DOI: 10.1002/rcs.1425. View

16.

Klunk W, Wang Y, Huang G, Debnath M, Holt D, Mathis C . Uncharged thioflavin-T derivatives bind to amyloid-beta protein with high affinity and readily enter the brain. Life Sci. 2001; 69(13):1471-84. DOI: 10.1016/s0024-3205(01)01232-2. View

17.

Kaneko S, Kaneko S . Fluorescence-Guided Resection of Malignant Glioma with 5-ALA. Int J Biomed Imaging. 2016; 2016:6135293. PMC: 4939363. DOI: 10.1155/2016/6135293. View

18.

Waters J . Accuracy and precision in quantitative fluorescence microscopy. J Cell Biol. 2009; 185(7):1135-48. PMC: 2712964. DOI: 10.1083/jcb.200903097. View

19.

Povazay B, Bizheva K, Unterhuber A, Hermann B, Sattmann H, Fercher A . Submicrometer axial resolution optical coherence tomography. Opt Lett. 2007; 27(20):1800-2. DOI: 10.1364/ol.27.001800. View

20.

Wilcock D, Gordon M, Morgan D . Quantification of cerebral amyloid angiopathy and parenchymal amyloid plaques with Congo red histochemical stain. Nat Protoc. 2007; 1(3):1591-5. DOI: 10.1038/nprot.2006.277. View