Non-destructive Characterization of Adult Zebrafish Models Using Jones Matrix Optical Coherence Tomography

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2022 May 6

PMID 35519284

Authors

Antonia Lichtenegger

Pradipta Mukherjee

Lida Zhu

Rion Morishita

Kiriko Tomita

Daisuke Oida

Konrad Leskovar

Ibrahim Abd El-Sadek

Shuichi Makita

Stefanie Kirchberger

Martin Distel

Bernhard Baumann

Yoshiaki Yasuno

Affiliations

Soon will be listed here.

Abstract

The zebrafish is a valuable vertebrate animal model in pre-clinical cancer research. A Jones matrix optical coherence tomography (JM-OCT) prototype operating at 1310 nm and an intensity-based spectral-domain OCT setup at 840 nm were utilized to investigate adult wildtype and a tumor-developing zebrafish model. Various anatomical features were characterized based on their inherent scattering and polarization signature. A motorized translation stage in combination with the JM-OCT prototype enabled large field-of-view imaging to investigate adult zebrafish in a non-destructive way. The diseased animals exhibited tumor-related abnormalities in the brain and near the eye region. The scatter intensity, the attenuation coefficients and local polarization parameters such as the birefringence and the degree of polarization uniformity were analyzed to quantify differences in tumor versus control regions. The proof-of-concept study in a limited number of animals revealed a significant decrease in birefringence in tumors found in the brain and near the eye compared to control regions. The presented work showed the potential of OCT and JM-OCT as non-destructive, high-resolution, and real-time imaging modalities for pre-clinical research based on zebrafish.

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