OptoPAD, a Closed-loop Optogenetics System to Study the Circuit Basis of Feeding Behaviors

Overview

Journal Elife

Specialty Biology

Date 2019 Jun 22

PMID 31226244

Citations 19

Authors

Jose-Maria Moreira

Pavel M Itskov

Dennis Goldschmidt

Celia Baltazar

Kathrin Steck

Ibrahim Tastekin

Samuel J Walker

Carlos Ribeiro

Affiliations

Soon will be listed here.

Abstract

The regulation of feeding plays a key role in determining the fitness of animals through its impact on nutrition. Elucidating the circuit basis of feeding and related behaviors is an important goal in neuroscience. We recently used a system for closed-loop optogenetic manipulation of neurons contingent on the feeding behavior of to dissect the impact of a specific subset of taste neurons on yeast feeding. Here, we describe the development and validation of this system, which we term the optoPAD. We use the optoPAD to induce appetitive and aversive effects on feeding by activating or inhibiting gustatory neurons in closed-loop - effectively creating virtual taste realities. The use of optogenetics allowed us to vary the dynamics and probability of stimulation in single flies and assess the impact on feeding behavior quantitatively and with high throughput. These data demonstrate that the optoPAD is a powerful tool to dissect the circuit basis of feeding behavior, allowing the efficient implementation of sophisticated behavioral paradigms to study the mechanistic basis of animals' adaptation to dynamic environments.

Citing Articles

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Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors.

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Selective integration of diverse taste inputs within a single taste modality.

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