Expression and Allele Frequencies of Thymic Stromal Lymphopoietin Are a Key Factor of Breast Cancer Risk

Overview

Journal Mol Genet Genomic Med

Specialty Genetics

Date 2019 Jun 19

PMID 31210014

Citations 6

Authors

Abdelhabib Semlali

Mikhlid Almutairi

Narasimha Reddy Parine

Abdullah Al Amri

Rafa Almeer

Mohammad S Alanazi

Mahmoud Rouabhia

Affiliations

Soon will be listed here.

Abstract

Background: Thymic stromal Lymphopoeitin (TSLP) is a key cytokine involved in inflammation and cancer progression. TSLP gene polymorphisms have been associated with increased susceptibility to cancer progression in different organs. We performed a control case study to examine the correlation of expression and polymorphisms of three nucleotides in TSLP with breast cancer (BC) risk in Saudi Arabian females.

Materials And Methods: The study was conducted on 116 healthy control subjects and 127 female patients with BC for the purpose of genotyping. Ten matching tissues provided data on immunohistochemistry to evaluate TSLP expression. Three SNPs (rs10043985, rs2289276, and rs3806933) were genotyped with TaqMan allelic discrimination assay. The patients' ages and estrogen receptor statuses were used to investigate the potential correlations between the different variations of TSLP genotypes and BC risk.

Results: BC tissues expressed positive immuno-staining for TSLP at a high rate compared to normal matching breast tissues. Malignant breast tumors exhibited higher TSLP expression than benign breast tumors. We also found that the rs3806933 (T) allele frequency decreased the risk of developing BC in the study population (OR = 0.356, p = 0.00027) significantly (0.356 times). Interestingly, statistical analysis revealed that the genotype mutant (AC) and the allele mutant (C) of rs10043985 within TSLP were significantly correlated with an increased BC risk (odds ratio [OR] = 4.762, confidence interval [CI] = 1.000-22.666, p = 0.03244; OR = 4.762, CI = 1.000-22.666, p = 0.03244; and OR = 4.575, CI = 0.975-21.464, p = 0.03516, respectively). In addition, the AC and AC + CC genotypes of TSLP rs10043985 were confirmed to be associated with an increased risk of BC risk in women aged above 48 years, compared with the AA genotype (AC and AC + CC vs. AA: OR = 9.468, CI = 0.493-181.768, p = 0.04537).

Conclusion: The results reveal significant correlation between SNPs in TSLP and BC progression in Saudi Arabian female patients.

Citing Articles

Development of a Novel Immune-Related Gene Prognostic Index for Breast Cancer.

Yao Y, Kong X, Liu R, Xu F, Liu G, Sun C Front Immunol. 2022; 13:845093.

PMID: 35558081 PMC: 9086776. DOI: 10.3389/fimmu.2022.845093.

Expression and Polymorphism of TSLP/TSLP Receptors as Potential Diagnostic Markers of Colorectal Cancer Progression.

Semlali A, Almutairi M, Alamri A, Reddy Parine N, Arafah M, Almadi M Genes (Basel). 2021; 12(9).

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CRLF2 and IKZF1 abnormalities in Mexican children with acute lymphoblastic leukemia and recurrent gene fusions: exploring surrogate markers of signaling pathways.

Moreno Lorenzana D, Juarez Velazquez M, Reyes Leon A, Martinez Anaya D, Hernandez Monterde A, Salas Labadia C J Pathol Clin Res. 2021; 7(4):410-421.

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The Combined Detection of Immune Genes for Predicting the Prognosis of Patients With Non-Small Cell Lung Cancer.

Tian W, Liu S, Li B Technol Cancer Res Treat. 2020; 19:1533033820977504.

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Modulation of Signaling Mediated by TSLP and IL-7 in Inflammation, Autoimmune Diseases, and Cancer.

Markovic I, Savvides S Front Immunol. 2020; 11:1557.

PMID: 32849527 PMC: 7396566. DOI: 10.3389/fimmu.2020.01557.

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