MicroRNA-101a Regulates Autophagy Phenomenon Via the MAPK Pathway to Modulate Alzheimer's-Associated Pathogenesis

Overview

Journal Cell Transplant

Specialties Cell Biology
General Surgery

Date 2019 Jun 18

PMID 31204500

Citations 23

Authors

Qian Li

Yu Wang

Wenjie Peng

Yanjie Jia

Jinhua Tang

WanWei Li

John H Zhang

Jun Yang

Affiliations

Soon will be listed here.

Abstract

Alzheimer's disease (AD) is a type of neurodegenerative disorder and the most common form of dementia. MicroRNA (miRNA) has been shown to play a role in various diseases, including AD. It also has been reported to regulate autophagy. We extracted miRNA from blood samples and constructed an miRNA-101a lentivirus vector. In this study we found the level of miRNA-101a was significantly reduced in the plasma of patients with AD and APPswe/PS1ΔE9 transgenic mice. The relative expression of miRNA-101a exhibited a relatively high diagnostic performance (area under receiver operating characteristic curve: 0.8725) in the prediction of AD with a sensitivity of 0.913 and a specificity of 0.733 at the threshold of 0.6463. Under electron microscopy, autophagic vacuoles in AD-related cells numbered more than the cells up-regulating miRNA-101a in the in vitro experiments. Dual-luciferase reporter assay and Western blot results proved that the MAPK1 pathway plays a role in the formation of autophagic vacuoles in AD. This study found that the autophagy phenomenon regulated by miRNA-101a via the MAPK pathway might be a new mechanism in AD. This could provide new insights into AD formation and treatment.

Citing Articles

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