Repeated Immunosensing by a Dithiobis(succinimidyl Propionate)-modified SAW Device

The possibility of dithiobis(succinimidyl propionate) (DSP) for repeated immunoassay with a surface acoustic wave (SAW) immunosensor was explored. In the sensor, DSP was used to modify a gold-coated quartz sensing area of a SAW device by forming a self-assembled monolayer on the gold surface. In a model sandwich assay using mouse (mIgG) and anti-mouse (a-mIgG) antibodies, the primary antibody, mIgG, firstly reacted with N-hydroxysuccinimide ester groups of DSP and was immobilized on the SAW device to fabricate the SAW immunosensor. Optimization of adsorption time of mIgG revealed that both degrees of adsorption and immobilization of mIgG reached a saturation at 30 min although the immobilization was more dependent on the adsorption time. Through characterization of the DSP-modified SAW immunosensor, a high selectivity, with which no sensor output was observed from various kinds of secondary antibodies except for a-mIgG, along with 8-fold shorter measurement time (15 min) than that of enzyme-linked immunosorbent assay were obtained. Furthermore, 10 repeated measurement of a-mIgG demonstrated a high reproducibility of the sensor output (coefficient of variation of 7.0%). These validate the utility of DSP in the SAW immunosensor for rapid and repeated measurement of antigens.